Cited 5 times in
Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 강신욱 | - |
dc.contributor.author | 김남희 | - |
dc.contributor.author | 김현실 | - |
dc.contributor.author | 박정탁 | - |
dc.contributor.author | 유태현 | - |
dc.contributor.author | 육종인 | - |
dc.contributor.author | 한승혁 | - |
dc.date.accessioned | 2020-12-11T08:06:49Z | - |
dc.date.available | 2020-12-11T08:06:49Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 1582-1838 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/180776 | - |
dc.description.abstract | While the bone morphogenetic protein-7 (BMP-7) is a well-known therapeutic growth factor reverting many fibrotic diseases, including peritoneal fibrosis by peritoneal dialysis (PD), soluble growth factors are largely limited in clinical applications owing to their short half-life in clinical settings. Recently, we developed a novel drug delivery model using protein transduction domains (PTD) overcoming limitation of soluble recombinant proteins, including bone morphogenetic protein-7 (BMP-7). This study aims at evaluating the therapeutic effects of PTD-BMP-7 consisted of PTD and full-length BMP-7 on epithelial-mesenchymal transition (EMT)-related fibrosis. Human peritoneal mesothelial cells (HPMCs) were then treated with TGF-β1 or TGF-β1 + PTD-BMP-7. Peritoneal dialysis (PD) catheters were inserted into Sprague-Dawley rats, and these rats were infused intra-peritoneally with saline, peritoneal dialysis fluid (PDF) or PDF + PTD-BMP-7. In vitro, TGF-β1 treatment significantly increased fibronectin, type I collagen, α-SMA and Snail expression, while reducing E-cadherin expression in HPMCs (P < .001). PTD-BMP-7 treatment ameliorated TGF-β1-induced fibronectin, type I collagen, α-SMA and Snail expression, and restored E-cadherin expression in HPMCs (P < .001). In vivo, the expressions of EMT-related molecules and the thickness of the sub-mesothelial layer were significantly increased in the peritoneum of rats treated with PDF, and these changes were significantly abrogated by the intra-peritoneal administration of PTD-BMP-7. PTD-BMP-7 treatment significantly inhibited the progression of established PD fibrosis. These findings suggest that PTD-BMP-7, as a prodrug of BMP-7, can be an effective therapeutic agent for peritoneal fibrosis in PD patients. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Seonghun Kim | - |
dc.contributor.googleauthor | Dong Ho Shin | - |
dc.contributor.googleauthor | Bo Young Nam | - |
dc.contributor.googleauthor | Hye-Young Kang | - |
dc.contributor.googleauthor | Jimin Park | - |
dc.contributor.googleauthor | Meiyan Wu | - |
dc.contributor.googleauthor | Nam Hee Kim | - |
dc.contributor.googleauthor | Hyun Sil Kim | - |
dc.contributor.googleauthor | Jung Tak Park | - |
dc.contributor.googleauthor | Seung Hyeok Han | - |
dc.contributor.googleauthor | Shin-Wook Kang | - |
dc.contributor.googleauthor | Jong In Yook | - |
dc.contributor.googleauthor | Tae-Hyun Yoo | - |
dc.identifier.doi | 10.1111/jcmm.15992 | - |
dc.contributor.localId | A00053 | - |
dc.contributor.localId | A00360 | - |
dc.contributor.localId | A01121 | - |
dc.contributor.localId | A01654 | - |
dc.contributor.localId | A02526 | - |
dc.contributor.localId | A02536 | - |
dc.contributor.localId | A04304 | - |
dc.relation.journalcode | J01302 | - |
dc.identifier.eissn | 1582-4934 | - |
dc.identifier.pmid | 33079436 | - |
dc.subject.keyword | PTD-BMP-7 | - |
dc.subject.keyword | and peritoneal fibrosis | - |
dc.subject.keyword | protein transduction domain | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.affiliatedAuthor | 강신욱 | - |
dc.contributor.affiliatedAuthor | 김남희 | - |
dc.contributor.affiliatedAuthor | 김현실 | - |
dc.contributor.affiliatedAuthor | 박정탁 | - |
dc.contributor.affiliatedAuthor | 유태현 | - |
dc.contributor.affiliatedAuthor | 육종인 | - |
dc.contributor.affiliatedAuthor | 한승혁 | - |
dc.citation.volume | 24 | - |
dc.citation.number | 22 | - |
dc.citation.startPage | 13507 | - |
dc.citation.endPage | 13522 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, Vol.24(22) : 13507-13522, 2020-10 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.