106 215

Cited 0 times in

FAM188B Expression Is Critical for Cell Growth via FOXM1 Regulation in Lung Cancer

Authors
 Young Eun Choi  ;  Hamadi Madhi  ;  HaEun Kim  ;  Jeon-Soo Lee  ;  Myung-Hee Kim  ;  Yong-Nyun Kim  ;  Sung-Ho Goh 
Citation
 BIOMEDICINES, Vol.8(11) : E465, 2020-10 
Journal Title
BIOMEDICINES
Issue Date
2020-10
Keywords
FAM188B ; FOXM1 ; cell survival ; deubiquitination ; lung cancer
Abstract
Although family with sequence similarity 188 member B (FAM188B) is known to be a member of a novel putative deubiquitinase family, its biological role has not been fully elucidated. Here, we demonstrate the oncogenic function of FAM188B via regulation of forkhead box M1 (FOXM1), another oncogenic transcription factor, in lung cancer cells. FAM188B knockdown induced the inhibition of cell growth along with the downregulation of mRNA and protein levels of FOXM1. FAM188B knockdown also resulted in downregulation of Survivin and cell cycle-related proteins, which are direct targets of FOXM1. Interestingly, FOXM1 co-immunoprecipitated with FAM188B, and the levels of FOXM1 ubiquitination increased with FAM188B knockdown but decreased with FAM188B overexpression. In addition, in vivo xenograft of FAM188B siRNA (siFAM188B) RNA-treated cells resulted in the retardation of tumor growth compared with that in the control. Furthermore, protein levels of FAM188B and FOXM1 were elevated in the human lung cancer tissues, and FAM188B expression was negatively correlated with the overall survival of lung cancer patients. These results indicate that FAM188B exerts its oncogenic effects by regulating FOXM1 deubiquitination and thus its stability. Therefore, FAM188B might be a potential therapeutic target to control lung cancer progression.
Files in This Item:
T202004924.pdf Download
DOI
10.3390/biomedicines8110465
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/180572
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links