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ER stress induced by ER calcium depletion and UVB irradiation regulates tight junction barrier integrity in human keratinocytes

DC FieldValueLanguage
dc.contributor.author이상은-
dc.date.accessioned2020-12-01T17:24:38Z-
dc.date.available2020-12-01T17:24:38Z-
dc.date.issued2020-04-
dc.identifier.issn0923-1811-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/180255-
dc.description.abstractBackground: Endoplasmic reticulum (ER) calcium depletion-induced ER stress is a crucial signal for keratinocyte differentiation and barrier homeostasis, but its effects on the epidermal tight junction (TJ) have not been characterized. Ultraviolet B (UVB) causes ER calcium release in keratinocytes and disrupts epidermal TJ, however, the involvement of ER stress in the UVB-induced TJ alterations remains unknown. Objectives: To investigate the effect of ER stress by pharmacological ER calcium depletion or UVB on the TJ integrity in normal human epidermal keratinocytes (NHEK). Methods: NHEK were exposed to ER calcium pump inhibitor thapsigargin (Tg) or UVB. ER stress markers and TJ molecules expression, TJ and F-actin structures, and TJ barrier function were analyzed. Results: Tg or UVB exposure dose-dependently triggered unfolded protein response (UPR) in NHEK. Low dose Tg induced the IRE1α-XBP1 pathway and strengthened TJ barrier. Contrary, high dose Tg activated PERK phosphorylation and disrupted TJ by F-actin disorganization. UVB disrupted TJ and F-actin structures dose dependently. IRE1α RNase inhibition induced or exacerbated TJ and F-actin disruption in the presence of low dose Tg or UVB. High dose Tg increased RhoA activity. 4-PBA or Rho kinase (ROCK) inhibitor partially prevented the disruption of TJ and F-actin following high dose Tg or UVB. Conclusions: ER stress has bimodal effects on the epidermal TJ depending on its intensity. The IRE1α pathway is critical for the maintenance of TJ integrity during mild ER stress. Severe ER stress-induced UPR or ROCK signalling mediates the disruption of TJ through cytoskeletal disorganization during severe ER stress.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfJOURNAL OF DERMATOLOGICAL SCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleER stress induced by ER calcium depletion and UVB irradiation regulates tight junction barrier integrity in human keratinocytes-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Dermatology (피부과학교실)-
dc.contributor.googleauthorSeong Hoon Seo-
dc.contributor.googleauthorSong-Ee Kim-
dc.contributor.googleauthorSang Eun Lee-
dc.identifier.doi10.1016/j.jdermsci.2020.02.006-
dc.contributor.localIdA02826-
dc.relation.journalcodeJ01370-
dc.identifier.eissn1873-569X-
dc.identifier.pmid32376153-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0923181120300773-
dc.subject.keywordBimodal effects-
dc.subject.keywordER stress-
dc.subject.keywordRho kinase-
dc.subject.keywordTight junction-
dc.subject.keywordUltraviolet B-
dc.subject.keywordUnfolded protein response-
dc.contributor.alternativeNameLee, Sang Eun-
dc.contributor.affiliatedAuthor이상은-
dc.citation.volume98-
dc.citation.number1-
dc.citation.startPage41-
dc.citation.endPage49-
dc.identifier.bibliographicCitationJOURNAL OF DERMATOLOGICAL SCIENCE, Vol.98(1) : 41-49, 2020-04-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers

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