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Chemical Controllable Gene Drive in Drosophila

Authors
 Dongwoo Chae  ;  Junwon Lee  ;  Nayoung Lee  ;  Kyungsoo Park  ;  Seok Jun Moon  ;  Hyongbum H Kim 
Citation
 ACS SYNTHETIC BIOLOGY, Vol.9(9) : 2362-2377, 2020-07 
Journal Title
 ACS SYNTHETIC BIOLOGY 
Issue Date
2020-07
Keywords
CRISPR-Cas9 system ; Rippase ; gene drive ; mutagenic chain reaction ; population genetics
Abstract
Gene drive systems that propagate transgenes via super-Mendelian inheritance can potentially control insect-borne diseases and agricultural pests. However, concerns have been raised regarding unforeseen ecological consequences, and methods that prevent undesirable gene drive effects have been proposed. Here, we report a chemical-induced control of gene drive. We prepared a CRISPR-based gene drive system that can be removed by a site-specific recombinase, Rippase, the expression of which is induced by the chemical RU486 in fruit flies. Exposure of fruit flies to RU486 resulted in 7-12% removal of gene drive elements at each generation, leading to a significant reduction in gene drive-fly propagation. Mathematical modeling and simulation suggest that our system offers several advantages over a previously reported gene drive control system. Our chemical control system can provide a proof-of-principle for the reversible control of gene drive effects depending on ecological status and human needs.
Full Text
https://pubs.acs.org/doi/10.1021/acssynbio.0c00117
DOI
10.1021/acssynbio.0c00117
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyongbum(김형범) ORCID logo https://orcid.org/0000-0002-4693-738X
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Park, Kyungsoo(박경수) ORCID logo https://orcid.org/0000-0002-6972-1143
Lee, Jun Won(이준원) ORCID logo https://orcid.org/0000-0003-0543-7132
Chae, Dong Woo(채동우) ORCID logo https://orcid.org/0000-0002-7675-3821
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/180230
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