Cited 7 times in
A Novel KPC Variant KPC-55 in Klebsiella pneumoniae ST307 of Reinforced Meropenem-Hydrolyzing Activity
DC Field | Value | Language |
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dc.contributor.author | 정석훈 | - |
dc.contributor.author | 최종락 | - |
dc.date.accessioned | 2020-12-01T17:18:24Z | - |
dc.date.available | 2020-12-01T17:18:24Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/180211 | - |
dc.description.abstract | A novel Klebsiella pneumoniae carbapenemase (KPC) variant, KPC-55, produced by a K. pneumoniae ST307 strain was characterized. K. pneumoniae strain BS407 was recovered from an active surveillance rectal swab of a patient newly admitted to a general hospital in Busan, South Korea. Carbapenemase production was confirmed by the modified Hodge test, and the MICs of β-lactams were determined by the broth microdilution method. The whole genome was sequenced. Cloning and expression of the bla KPC-55 gene in Escherichia coli and MIC determination were performed. The enzyme KPC-55 was used for kinetic assays against β-lactams and compared with the KPC-2 enzyme. The new allele of the bla KPC gene had a T794A alteration compared to the bla KPC-2 gene, resulting in the amino acid substitution Y264N in the middle of the β9-sheet. Compared to the KPC-2-producing strain, the KPC-55-producing strain exhibited a lower level of resistance to most β-lactam drugs tested, however, the KPC-55 enzyme catalyzed aztreonam and meropenem at an increased efficiency compared to the catalytic activity of KPC-2. KPC subtypes could have varied phenotypes due to alterations in amino acid sequences, and such an unexpected resistance phenotype emphasizes the importance of detailed characterizations for the carbapenemase-producing Enterobacterales. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Frontiers Research Foundation | - |
dc.relation.isPartOf | FRONTIERS IN MICROBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | A Novel KPC Variant KPC-55 in Klebsiella pneumoniae ST307 of Reinforced Meropenem-Hydrolyzing Activity | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Eun-Jeong Yoon | - |
dc.contributor.googleauthor | You Jeong Choi | - |
dc.contributor.googleauthor | Sun Hee Park | - |
dc.contributor.googleauthor | Jeong Hwan Shin | - |
dc.contributor.googleauthor | Sung Gyun Park | - |
dc.contributor.googleauthor | Jong Rak Choi | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.identifier.doi | 10.3389/fmicb.2020.561317 | - |
dc.contributor.localId | A03619 | - |
dc.contributor.localId | A04182 | - |
dc.relation.journalcode | J03413 | - |
dc.identifier.eissn | 1664-302X | - |
dc.identifier.pmid | 33133036 | - |
dc.subject.keyword | KPC-55 | - |
dc.subject.keyword | Klebsiella pneumoniae | - |
dc.subject.keyword | ST307 | - |
dc.subject.keyword | carbapenemase-producing Enterobacterales | - |
dc.subject.keyword | meropenem | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | 정석훈 | - |
dc.contributor.affiliatedAuthor | 최종락 | - |
dc.citation.volume | 11 | - |
dc.citation.startPage | 561317 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN MICROBIOLOGY, Vol.11 : 561317, 2020-10 | - |
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