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Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in Klebsiella pneumoniae for Stable Propagation

DC FieldValueLanguage
dc.contributor.author김도균-
dc.contributor.author원동주-
dc.contributor.author유창승-
dc.contributor.author정석훈-
dc.contributor.author최종락-
dc.date.accessioned2020-12-01T17:18:06Z-
dc.date.available2020-12-01T17:18:06Z-
dc.date.issued2020-10-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/180210-
dc.description.abstractThe acquired CTX-M-type extended-spectrum-β-lactamase (ESBL)-producing Enterobacterales are of great concern in clinical settings because they limit therapeutic options for patients infected by the pathogens. An intriguing clonality of CTX-M ESBL-producing Klebsiella pneumoniae blood isolates was observed from a national cohort study, and comparative genomics were assessed for the 115 K. pneumoniae blood isolates carrying the bla CTX-M gene. The plasmid preference of particular clones of a sequence type (ST) was assessed by liquid mating. A quarter of the bla CTX-M gene-carrying K. pneumoniae blood isolates harbor the gene in their chromosome, and most of those with the built-in bla CTX-M gene belonged either to ST307 or ST48. Notably, all 16 K. pneumoniae ST48 isolates harbored two copies of the bla CTX-M-15 gene in the chromosome. The chromosomal integration of the bla CTX-M-15 gene was mostly derived from the ISEcp1-targeting 5-bp AT-rich locus in the chromosome. The IS26-mediated chromosomal integration occurred when the upstream ISEcp1 from the bla CTX-M gene was truncated, targeting the anchor IS26 copy in the chromosome. Higher transfer efficiency of the bla CTX-M-15 gene-carrying FIA:R plasmid was observed in ST17 than that of the bla CTX-M-14 gene-carrying FIB:FII plasmid. The transfer efficiency of the plasmid differed by isolate among the ST307 members. The K. pneumoniae clones ST307 and ST48 harboring the bla CTX-M-15 gene in the chromosome were able to disseminate stably in clinical settings regardless of the environmental pressure, and the current population of K. pneumoniae blood isolates was constructed. Further follow-up is needed for the epidemiology of this antimicrobial resistance.IMPORTANCE Dominant F-type plasmids harboring the gene have been pointed out to be responsible for the dissemination of the CTX-M extended-spectrum-β-lactamase (ESBL)-producing K. pneumoniae Recently, the emergence of K. pneumoniae isolates with the bla CTX-M gene in their chromosomes has been reported occasionally worldwide. Such a chromosomal location of the resistance gene could be beneficial for stable propagation, as was the Acinetobacter baumannii ST191 harboring chromosomal bla OXA-23 that is endemic to South Korea. Through the present study, particular clones were identified as having built-in resistance genes in their chromosomes, and the chromosomal integration events were tracked by assessing their genomes. The cefotaxime-resistant K. pneumoniae clones of this study were particularized as results of the fastidiousness for plasmids to acquire the bla CTX-M gene for securing the diversity and of the chromosomal addiction of the bla CTX-M gene for ensuring propagation.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherAmerican Society for Microbiology-
dc.relation.isPartOfMSYSTEMS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleBeneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in Klebsiella pneumoniae for Stable Propagation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.googleauthorEun-Jeong Yoon-
dc.contributor.googleauthorBareum Gwon-
dc.contributor.googleauthorChangseung Liu-
dc.contributor.googleauthorDokyun Kim-
dc.contributor.googleauthorDongju Won-
dc.contributor.googleauthorSung Gyun Park-
dc.contributor.googleauthorJong Rak Choi-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.identifier.doi10.1128/mSystems.00459-20-
dc.contributor.localIdA04891-
dc.contributor.localIdA05763-
dc.contributor.localIdA05763-
dc.contributor.localIdA05704-
dc.contributor.localIdA05704-
dc.contributor.localIdA03619-
dc.contributor.localIdA03619-
dc.contributor.localIdA04182-
dc.contributor.localIdA04182-
dc.relation.journalcodeJ03628-
dc.identifier.eissn2379-5077-
dc.identifier.pmid32994286-
dc.subject.keywordCTX-M-
dc.subject.keywordIS26-
dc.subject.keywordISEcp1-
dc.subject.keywordKlebsiella pneumoniae-
dc.subject.keywordchromosomal integration-
dc.subject.keywordextended-spectrum β-lactamases-
dc.contributor.alternativeNameKim, Dokyun-
dc.contributor.affiliatedAuthor김도균-
dc.contributor.affiliatedAuthor원동주-
dc.contributor.affiliatedAuthor원동주-
dc.contributor.affiliatedAuthor유창승-
dc.contributor.affiliatedAuthor유창승-
dc.contributor.affiliatedAuthor정석훈-
dc.contributor.affiliatedAuthor정석훈-
dc.contributor.affiliatedAuthor최종락-
dc.contributor.affiliatedAuthor최종락-
dc.citation.volume5-
dc.citation.number5-
dc.citation.startPagee00459-20-
dc.identifier.bibliographicCitationMSYSTEMS, Vol.5(5) : e00459-20, 2020-10-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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