Cited 26 times in
Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in Klebsiella pneumoniae for Stable Propagation
DC Field | Value | Language |
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dc.contributor.author | 김도균 | - |
dc.contributor.author | 원동주 | - |
dc.contributor.author | 유창승 | - |
dc.contributor.author | 정석훈 | - |
dc.contributor.author | 최종락 | - |
dc.date.accessioned | 2020-12-01T17:18:06Z | - |
dc.date.available | 2020-12-01T17:18:06Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/180210 | - |
dc.description.abstract | The acquired CTX-M-type extended-spectrum-β-lactamase (ESBL)-producing Enterobacterales are of great concern in clinical settings because they limit therapeutic options for patients infected by the pathogens. An intriguing clonality of CTX-M ESBL-producing Klebsiella pneumoniae blood isolates was observed from a national cohort study, and comparative genomics were assessed for the 115 K. pneumoniae blood isolates carrying the bla CTX-M gene. The plasmid preference of particular clones of a sequence type (ST) was assessed by liquid mating. A quarter of the bla CTX-M gene-carrying K. pneumoniae blood isolates harbor the gene in their chromosome, and most of those with the built-in bla CTX-M gene belonged either to ST307 or ST48. Notably, all 16 K. pneumoniae ST48 isolates harbored two copies of the bla CTX-M-15 gene in the chromosome. The chromosomal integration of the bla CTX-M-15 gene was mostly derived from the ISEcp1-targeting 5-bp AT-rich locus in the chromosome. The IS26-mediated chromosomal integration occurred when the upstream ISEcp1 from the bla CTX-M gene was truncated, targeting the anchor IS26 copy in the chromosome. Higher transfer efficiency of the bla CTX-M-15 gene-carrying FIA:R plasmid was observed in ST17 than that of the bla CTX-M-14 gene-carrying FIB:FII plasmid. The transfer efficiency of the plasmid differed by isolate among the ST307 members. The K. pneumoniae clones ST307 and ST48 harboring the bla CTX-M-15 gene in the chromosome were able to disseminate stably in clinical settings regardless of the environmental pressure, and the current population of K. pneumoniae blood isolates was constructed. Further follow-up is needed for the epidemiology of this antimicrobial resistance.IMPORTANCE Dominant F-type plasmids harboring the gene have been pointed out to be responsible for the dissemination of the CTX-M extended-spectrum-β-lactamase (ESBL)-producing K. pneumoniae Recently, the emergence of K. pneumoniae isolates with the bla CTX-M gene in their chromosomes has been reported occasionally worldwide. Such a chromosomal location of the resistance gene could be beneficial for stable propagation, as was the Acinetobacter baumannii ST191 harboring chromosomal bla OXA-23 that is endemic to South Korea. Through the present study, particular clones were identified as having built-in resistance genes in their chromosomes, and the chromosomal integration events were tracked by assessing their genomes. The cefotaxime-resistant K. pneumoniae clones of this study were particularized as results of the fastidiousness for plasmids to acquire the bla CTX-M gene for securing the diversity and of the chromosomal addiction of the bla CTX-M gene for ensuring propagation. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isPartOf | MSYSTEMS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in Klebsiella pneumoniae for Stable Propagation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Eun-Jeong Yoon | - |
dc.contributor.googleauthor | Bareum Gwon | - |
dc.contributor.googleauthor | Changseung Liu | - |
dc.contributor.googleauthor | Dokyun Kim | - |
dc.contributor.googleauthor | Dongju Won | - |
dc.contributor.googleauthor | Sung Gyun Park | - |
dc.contributor.googleauthor | Jong Rak Choi | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.identifier.doi | 10.1128/mSystems.00459-20 | - |
dc.contributor.localId | A04891 | - |
dc.contributor.localId | A05763 | - |
dc.contributor.localId | A05704 | - |
dc.contributor.localId | A03619 | - |
dc.contributor.localId | A04182 | - |
dc.relation.journalcode | J03628 | - |
dc.identifier.eissn | 2379-5077 | - |
dc.identifier.pmid | 32994286 | - |
dc.subject.keyword | CTX-M | - |
dc.subject.keyword | IS26 | - |
dc.subject.keyword | ISEcp1 | - |
dc.subject.keyword | Klebsiella pneumoniae | - |
dc.subject.keyword | chromosomal integration | - |
dc.subject.keyword | extended-spectrum β-lactamases | - |
dc.contributor.alternativeName | Kim, Dokyun | - |
dc.contributor.affiliatedAuthor | 김도균 | - |
dc.contributor.affiliatedAuthor | 원동주 | - |
dc.contributor.affiliatedAuthor | 유창승 | - |
dc.contributor.affiliatedAuthor | 정석훈 | - |
dc.contributor.affiliatedAuthor | 최종락 | - |
dc.citation.volume | 5 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | e00459-20 | - |
dc.identifier.bibliographicCitation | MSYSTEMS, Vol.5(5) : e00459-20, 2020-10 | - |
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