Cited 3 times in
Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells
DC Field | Value | Language |
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dc.contributor.author | 윤미진 | - |
dc.date.accessioned | 2020-12-01T16:47:51Z | - |
dc.date.available | 2020-12-01T16:47:51Z | - |
dc.date.issued | 2020-09 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/179980 | - |
dc.description.abstract | In this work, the influence of parylene N film on the spheroid formation of osteoblast-like cells (MG-63) was determined and compared with that of high-hydrophilicity microenvironments, such as hydrophilic culture matrix and ultraviolet-treated parylene N film. To elucidate the change in cell properties due to the microenvironment of parylene N film, global gene expression profiles of MG-63 cells on parylene N film were analyzed. We confirmed the upregulated expression of osteoblast differentiation- and proliferation-related genes, such as Runx2, ALPL, and BGLAP and MKi67 and PCNA, respectively, using the real-time polymerase chain reaction. In addition, the differentiation and proliferation of osteoblast cells cultured on parylene N film were validated using immunostaining. Finally, the formation of spheroids and regulation of differentiation in human mesenchymal stem cells (MSCs) on parylene N film was demonstrated. The results of this study confirm that the microenvironment with the controlled hydrophobic property of parylene N film could effectively trigger the bone differentiation and maintains the proliferation of MSCs, similar to MG-63 cells without any scaffold structures or physical treatments. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Nuclear Medicine (핵의학교실) | - |
dc.contributor.googleauthor | Tae-Hun Kim | - |
dc.contributor.googleauthor | Jong-Sook Lee | - |
dc.contributor.googleauthor | Hanhee Jo | - |
dc.contributor.googleauthor | Yusun Park | - |
dc.contributor.googleauthor | Mijin Yun | - |
dc.contributor.googleauthor | Zhiquan Song | - |
dc.contributor.googleauthor | Jae-Chul Pyun | - |
dc.contributor.googleauthor | Misu Lee | - |
dc.identifier.doi | 10.1038/s41598-020-71322-1 | - |
dc.contributor.localId | A02550 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 32938961 | - |
dc.contributor.alternativeName | Yun, Mi Jin | - |
dc.contributor.affiliatedAuthor | 윤미진 | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 15219 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.10(1) : 15219, 2020-09 | - |
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