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Matrix metalloproteinase 9-activatable peptide-conjugated hydrogel-based fluorogenic intraocular-lens sensor

Authors
 Moo-Kwang Shin  ;  Yong Woo Ji  ;  Chae-Eun Moon  ;  Hyo Lee  ;  Byunghoon Kang  ;  Woo-Seok Jinn  ;  Jisun Ki  ;  Byunggeol Mun  ;  Myeong-Hoon Kim  ;  Hyung Keun Lee  ;  Seungjoo Haam 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.162 : 112254, 2020-08 
Journal Title
BIOSENSORS & BIOELECTRONICS
ISSN
 0956-5663 
Issue Date
2020-08
Keywords
Hydrogel sensor ; Intraocular-lens sensor ; Liquid biopsy ; Matrix metalloproteinase-9 ; Peptide-probe
Abstract
The eye is an extension of the central nervous system (CNS) and contains aqueous humor (AH), which is a fluid rich in biomolecules secreted from intraocular tissues; thus, this organ allows for non-invasive visualization of early changes in CNS disorders. There is a growing interest in developing implantable devices, such as intraocular-lens (IOL), for specific medical uses, including intraocular monitoring. We describe a novel IOL-sensing system for detecting AH biomarkers via biocompatible enzyme-activatable fluorogenic hydrogel sensors. Matrix-metalloproteinase-9, a biomarker of degenerative CNS and eye disorders, was selected as a target. A peptide-probe-incorporated fluorogenic IOL (FIOL) was developed using diacrylamide-group-modified poly(ethyleneglycol) (PEGDAAm) biocompatible hydrogels, adjusting the hydrogel mesh size to allow selective penetration of the target while blocking non-targets, using label-free detection with semi-permanently implantable sensors, and demonstrating the clinical feasibility of FIOL through in vivo testing. This novel FIOL-based sensing system represents a promising approach for liquid biopsy of intraocular fluids.
Full Text
https://www.sciencedirect.com/science/article/pii/S0956566320302505
DOI
10.1016/j.bios.2020.112254
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Hyung Keun(이형근) ORCID logo https://orcid.org/0000-0002-1123-2136
Ji, Yong Woo(지용우) ORCID logo https://orcid.org/0000-0002-7211-6278
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179690
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