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Identification of Fungal Species and Detection of Azole-Resistance Mutations in the Aspergillus fumigatus cyp51A Gene at a South Korean Hospital

DC FieldValueLanguage
dc.contributor.author염준섭-
dc.contributor.author손유진-
dc.contributor.author박윤수-
dc.contributor.author구남수-
dc.contributor.author김무현-
dc.contributor.author김정호-
dc.contributor.author정인영-
dc.contributor.author정수진-
dc.contributor.author현종훈-
dc.contributor.author손유진-
dc.contributor.author박윤수-
dc.contributor.author구남수-
dc.contributor.author김무현-
dc.contributor.author김정호-
dc.contributor.author정인영-
dc.contributor.author정수진-
dc.contributor.author현종훈-
dc.contributor.author최준용-
dc.contributor.author조윤숙-
dc.contributor.author백예지-
dc.contributor.author안진영-
dc.contributor.author심효섭-
dc.contributor.author손유진-
dc.contributor.author박윤수-
dc.contributor.author구남수-
dc.contributor.author김무현-
dc.contributor.author김정호-
dc.contributor.author정인영-
dc.contributor.author정수진-
dc.contributor.author현종훈-
dc.contributor.author최준용-
dc.contributor.author조윤숙-
dc.contributor.author백예지-
dc.contributor.author안진영-
dc.contributor.author심효섭-
dc.date.accessioned2020-10-03T16:48:14Z-
dc.date.available2020-10-03T16:48:14Z-
dc.date.issued2020-08-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/179657-
dc.description.abstractPurpose: With changing fungal epidemiology and azole resistance in Aspergillus species, identifying fungal species and susceptibility patterns is crucial to the management of aspergillosis and mucormycosis. The objectives of this study were to evaluate performance of panfungal polymerase chain reaction (PCR) assays on formalin-fixed paraffin embedded (FFPE) samples in the identification of fungal species and in the detection of azole-resistance mutations in the Aspergillus fumigatus cyp51A gene at a South Korean hospital. Materials and methods: A total of 75 FFPE specimens with a histopathological diagnosis of aspergillosis or mucormycosis were identified during the 10-year study period (2006-2015). After deparaffinization and DNA extraction, panfungal PCR assays were conducted on FFPE samples for fungal species identification. The identified fungal species were compared with histopathological diagnosis. On samples identified as A. fumigatus, sequencing to identify frequent mutations in the cyp51A gene [tandem repeat 46 (TR46), L98H, and M220 alterations] that confer azole resistance was performed. Results: Specific fungal DNA was identified in 31 (41.3%) FFPE samples, and of these, 16 samples of specific fungal DNA were in accord with a histopathological diagnosis of aspergillosis or mucormycosis; 15 samples had discordant histopathology and PCR results. No azole-mediating cyp51A gene mutation was noted among nine cases of aspergillosis. Moreover, no cyp51A mutations were identified among three cases with history of prior azole use. Conclusion: Panfungal PCR assay with FFPE samples may provide additional information of use to fungal species identification. No azole-resistance mediating mutations in the A. fumigatus cyp51A gene were identified among FFPE samples during study period.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAged-
dc.subject.MESHAged, 80 and over-
dc.subject.MESHAspergillus fumigatus / drug effects-
dc.subject.MESHAspergillus fumigatus / enzymology*-
dc.subject.MESHAspergillus fumigatus / genetics*-
dc.subject.MESHAzoles / pharmacology*-
dc.subject.MESHCytochrome P-450 Enzyme System / genetics*-
dc.subject.MESHDrug Resistance, Fungal / drug effects-
dc.subject.MESHDrug Resistance, Fungal / genetics*-
dc.subject.MESHFemale-
dc.subject.MESHHospitals*-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMicrobial Sensitivity Tests-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMutation / genetics*-
dc.subject.MESHRepublic of Korea-
dc.titleIdentification of Fungal Species and Detection of Azole-Resistance Mutations in the Aspergillus fumigatus cyp51A Gene at a South Korean Hospital-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorIn Young Jung-
dc.contributor.googleauthorYoun Jung Lee-
dc.contributor.googleauthorHyo Sup Shim-
dc.contributor.googleauthorYun Suk Cho-
dc.contributor.googleauthorYu Jin Sohn-
dc.contributor.googleauthorJong Hoon Hyun-
dc.contributor.googleauthorYae Jee Baek-
dc.contributor.googleauthorMoo Hyun Kim-
dc.contributor.googleauthorJung Ho Kim-
dc.contributor.googleauthorJin Young Ahn-
dc.contributor.googleauthorSu Jin Jeong-
dc.contributor.googleauthorNam Su Ku-
dc.contributor.googleauthorYoon Soo Park-
dc.contributor.googleauthorJoon Sup Yeom-
dc.contributor.googleauthorYoung Keun Kim-
dc.contributor.googleauthorHyo Youl Kim-
dc.contributor.googleauthorJun Yong Choi-
dc.identifier.doi10.3349/ymj.2020.61.8.698-
dc.contributor.localIdA02353-
dc.contributor.localIdA05924-
dc.contributor.localIdA05924-
dc.contributor.localIdA01598-
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dc.contributor.localIdA01598-
dc.contributor.localIdA01598-
dc.contributor.localIdA00189-
dc.contributor.localIdA00189-
dc.contributor.localIdA04521-
dc.contributor.localIdA04521-
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dc.contributor.localIdA00902-
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dc.contributor.localIdA03695-
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dc.contributor.localIdA04191-
dc.contributor.localIdA04191-
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dc.contributor.localIdA05921-
dc.contributor.localIdA05921-
dc.contributor.localIdA02267-
dc.contributor.localIdA02267-
dc.contributor.localIdA02219-
dc.contributor.localIdA02219-
dc.contributor.localIdA05924-
dc.contributor.localIdA05924-
dc.contributor.localIdA01598-
dc.contributor.localIdA01598-
dc.contributor.localIdA00189-
dc.contributor.localIdA00189-
dc.contributor.localIdA04521-
dc.contributor.localIdA04521-
dc.contributor.localIdA00902-
dc.contributor.localIdA00902-
dc.contributor.localIdA03695-
dc.contributor.localIdA03695-
dc.contributor.localIdA03638-
dc.contributor.localIdA03638-
dc.contributor.localIdA05944-
dc.contributor.localIdA05944-
dc.contributor.localIdA04191-
dc.contributor.localIdA04191-
dc.contributor.localIdA05940-
dc.contributor.localIdA05940-
dc.contributor.localIdA05921-
dc.contributor.localIdA05921-
dc.contributor.localIdA02267-
dc.contributor.localIdA02267-
dc.contributor.localIdA02219-
dc.contributor.localIdA02219-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid32734733-
dc.subject.keywordAspergillosis-
dc.subject.keywordfungus-
dc.subject.keywordmucormycosis-
dc.subject.keywordpolymerase chain reaction-
dc.subject.keywordresistance-
dc.contributor.alternativeNameYeom, Joon Sup-
dc.contributor.affiliatedAuthor염준섭-
dc.contributor.affiliatedAuthor손유진-
dc.contributor.affiliatedAuthor손유진-
dc.contributor.affiliatedAuthor박윤수-
dc.contributor.affiliatedAuthor박윤수-
dc.contributor.affiliatedAuthor구남수-
dc.contributor.affiliatedAuthor구남수-
dc.contributor.affiliatedAuthor김무현-
dc.contributor.affiliatedAuthor김무현-
dc.contributor.affiliatedAuthor김정호-
dc.contributor.affiliatedAuthor김정호-
dc.contributor.affiliatedAuthor정인영-
dc.contributor.affiliatedAuthor정인영-
dc.contributor.affiliatedAuthor정수진-
dc.contributor.affiliatedAuthor정수진-
dc.contributor.affiliatedAuthor현종훈-
dc.contributor.affiliatedAuthor현종훈-
dc.contributor.affiliatedAuthor손유진-
dc.contributor.affiliatedAuthor손유진-
dc.contributor.affiliatedAuthor박윤수-
dc.contributor.affiliatedAuthor박윤수-
dc.contributor.affiliatedAuthor구남수-
dc.contributor.affiliatedAuthor구남수-
dc.contributor.affiliatedAuthor김무현-
dc.contributor.affiliatedAuthor김무현-
dc.contributor.affiliatedAuthor김정호-
dc.contributor.affiliatedAuthor김정호-
dc.contributor.affiliatedAuthor정인영-
dc.contributor.affiliatedAuthor정인영-
dc.contributor.affiliatedAuthor정수진-
dc.contributor.affiliatedAuthor정수진-
dc.contributor.affiliatedAuthor현종훈-
dc.contributor.affiliatedAuthor현종훈-
dc.contributor.affiliatedAuthor최준용-
dc.contributor.affiliatedAuthor최준용-
dc.contributor.affiliatedAuthor조윤숙-
dc.contributor.affiliatedAuthor조윤숙-
dc.contributor.affiliatedAuthor백예지-
dc.contributor.affiliatedAuthor백예지-
dc.contributor.affiliatedAuthor안진영-
dc.contributor.affiliatedAuthor안진영-
dc.contributor.affiliatedAuthor심효섭-
dc.contributor.affiliatedAuthor심효섭-
dc.contributor.affiliatedAuthor손유진-
dc.contributor.affiliatedAuthor손유진-
dc.contributor.affiliatedAuthor박윤수-
dc.contributor.affiliatedAuthor박윤수-
dc.contributor.affiliatedAuthor구남수-
dc.contributor.affiliatedAuthor구남수-
dc.contributor.affiliatedAuthor김무현-
dc.contributor.affiliatedAuthor김무현-
dc.contributor.affiliatedAuthor김정호-
dc.contributor.affiliatedAuthor김정호-
dc.contributor.affiliatedAuthor정인영-
dc.contributor.affiliatedAuthor정인영-
dc.contributor.affiliatedAuthor정수진-
dc.contributor.affiliatedAuthor정수진-
dc.contributor.affiliatedAuthor현종훈-
dc.contributor.affiliatedAuthor현종훈-
dc.contributor.affiliatedAuthor최준용-
dc.contributor.affiliatedAuthor최준용-
dc.contributor.affiliatedAuthor조윤숙-
dc.contributor.affiliatedAuthor조윤숙-
dc.contributor.affiliatedAuthor백예지-
dc.contributor.affiliatedAuthor백예지-
dc.contributor.affiliatedAuthor안진영-
dc.contributor.affiliatedAuthor안진영-
dc.contributor.affiliatedAuthor심효섭-
dc.contributor.affiliatedAuthor심효섭-
dc.citation.volume61-
dc.citation.number8-
dc.citation.startPage698-
dc.citation.endPage704-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.61(8) : 698-704, 2020-08-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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