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ID1-Mediated BMP Signaling Pathway Potentiates Glucagon-Like Peptide-1 Secretion in Response to Nutrient Replenishment

DC FieldValueLanguage
dc.contributor.author이해경-
dc.contributor.author황성순-
dc.date.accessioned2020-09-28T11:32:27Z-
dc.date.available2020-09-28T11:32:27Z-
dc.date.issued2020-05-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/179222-
dc.description.abstractGlucagon-like peptide-1 (GLP-1) is a well-known incretin hormone secreted from enteroendocrinal L cells in response to nutrients, such as glucose and dietary fat, and controls glycemic homeostasis. However, the detailed intracellular mechanisms of how L cells control GLP-1 secretion in response to nutrients still remain unclear. Here, we report that bone morphogenetic protein (BMP) signaling pathway plays a pivotal role to control GLP-1 secretion in response to nutrient replenishment in well-established mouse enteroendocrinal L cells (GLUTag cells). Nutrient starvation dramatically reduced cellular respiration and GLP-1 secretion in GLUTag cells. Transcriptome analysis revealed that nutrient starvation remarkably reduced gene expressions involved in BMP signaling pathway, whereas nutrient replenishment rescued BMP signaling to potentiate GLP-1 secretion. Transient knockdown of inhibitor of DNA binding (ID)1, a well-known target gene of BMP signaling, remarkably reduced GLP-1 secretion. Consistently, LDN193189, an inhibitor of BMP signaling, markedly reduced GLP-1 secretion in L cells. In contrast, BMP4 treatment activated BMP signaling pathway and potentiated GLP-1 secretion in response to nutrient replenishment. Altogether, we demonstrated that BMP signaling pathway is a novel molecular mechanism to control GLP-1 secretion in response to cellular nutrient status. Selective activation of BMP signaling would be a potent therapeutic strategy to stimulate GLP-1 secretion in order to restore glycemic homeostasis.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherMDPI-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleID1-Mediated BMP Signaling Pathway Potentiates Glucagon-Like Peptide-1 Secretion in Response to Nutrient Replenishment-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentBioMedical Science Institute (의생명과학부)-
dc.contributor.googleauthorJae Woong Jeong-
dc.contributor.googleauthorMinki Kim-
dc.contributor.googleauthorJiwoo Lee-
dc.contributor.googleauthorHae-Kyung Lee-
dc.contributor.googleauthorYounhee Ko-
dc.contributor.googleauthorHyunkyung Kim-
dc.contributor.googleauthorSungsoon Fang-
dc.identifier.doi10.3390/ijms21113824-
dc.contributor.localIdA05936-
dc.contributor.localIdA05443-
dc.contributor.localIdA05443-
dc.relation.journalcodeJ01133-
dc.identifier.eissn1422-0067-
dc.identifier.pmid32481541-
dc.subject.keywordL cells-
dc.subject.keywordbone morphogenetic protein 4-
dc.subject.keywordglucagon-like peptide-1-
dc.subject.keywordincretin-
dc.subject.keywordinhibitor of DNA binding 1-
dc.contributor.alternativeNameLee, Hae-Kyung-
dc.contributor.affiliatedAuthor이해경-
dc.contributor.affiliatedAuthor황성순-
dc.contributor.affiliatedAuthor황성순-
dc.citation.volume21-
dc.citation.number11-
dc.citation.startPage3824-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.21(11) : 3824, 2020-05-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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