Cited 4 times in
Evaluation of Xpert Carba-R Assay v.2 to Detect Carbapenemase Genes in Two Hospitals in Korea
DC Field | Value | Language |
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dc.contributor.author | 최준용 | - |
dc.contributor.author | 용동은 | - |
dc.date.accessioned | 2020-09-28T11:29:59Z | - |
dc.date.available | 2020-09-28T11:29:59Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/179208 | - |
dc.description.abstract | Background: As the spread of carbapenemase-producing Enterobacteriaceae poses a critical threat to public health, rapid detection of carbapenemase genes is urgently required for prompt initiation of appropriate antimicrobial therapy and infection control. We evaluated the performance of Xpert Carba-R v.2 (Cepheid, USA) compared with that of culture-based conventional PCR. Methods: Using the results of 5,479 consecutive clinical rectal swabs, discrepant analysis (enriched culture followed by PCR) was performed for all discordant samples (N=100), which were Carba-R v.2-positive and culture-negative. Results: Among the samples, 206 carbapenemase genes (3.6%) were detected by Carba-R v.2. The sensitivity and specificity were 95.0% and 98.1%, respectively. The positive predictive value (PPV) and negative predictive value (NPV) were 49.0% and 99.9%, respectively. Following discrepant analysis, the PPV increased to 73.5% and the low PPV (8.1%) of the 86 non-KPC improved to 48.8%. Among the 105 discrepancies, NDM was the most frequently observed (N=56), followed by KPC (N=26), VIM (N=10), IMP (N=8), OXA-48 (N=5). The threshold cycle values between discordant vs. concordant and resolved groups were significantly different (P<0.001). Conclusions: Carba-R v.2 is a rapid and sensitive method for detecting carbapenemase-encoding genes compared with culture-based conventional PCR. Most of our discrepant results were non-KPC genes. Thus, the clinical significance of the non-KPC positive cases detected by Carba-R v.2 should be investigated. This assay would be useful for deciding whether to isolate pre-exposed patients in hospital settings, based on the high specificity and NPV. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Korean Society for Laboratory Medicine | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Evaluation of Xpert Carba-R Assay v.2 to Detect Carbapenemase Genes in Two Hospitals in Korea | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Jung Hyun Byun | - |
dc.contributor.googleauthor | Young Ah Kim | - |
dc.contributor.googleauthor | Milee Kim | - |
dc.contributor.googleauthor | Bomi Kim | - |
dc.contributor.googleauthor | Jun Yong Choi | - |
dc.contributor.googleauthor | Yoon Soo Park | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.identifier.doi | 10.3343/alm.2020.40.3.209 | - |
dc.contributor.localId | A04191 | - |
dc.contributor.localId | A02423 | - |
dc.relation.journalcode | J00164 | - |
dc.identifier.eissn | 2234-3814 | - |
dc.identifier.pmid | 31858760 | - |
dc.subject.keyword | Carbapenemase-producing Enterobacteriaceae | - |
dc.subject.keyword | Infection control | - |
dc.subject.keyword | KPC | - |
dc.subject.keyword | NDM | - |
dc.subject.keyword | Performance | - |
dc.subject.keyword | Xpert Carba-R v.2 | - |
dc.contributor.alternativeName | Choi, Jun Yong | - |
dc.contributor.affiliatedAuthor | 최준용 | - |
dc.contributor.affiliatedAuthor | 용동은 | - |
dc.citation.volume | 40 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 209 | - |
dc.citation.endPage | 215 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, Vol.40(3) : 209-215, 2020-05 | - |
dc.identifier.rimsid | 67441 | - |
dc.type.rims | ART | - |
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