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Therapeutic mechanism of cord blood mononuclear cells via the IL-8-mediated angiogenic pathway in neonatal hypoxic-ischaemic brain injury

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dc.contributor.author김동욱-
dc.date.accessioned2020-09-28T01:29:55Z-
dc.date.available2020-09-28T01:29:55Z-
dc.date.issued2020-03-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/179026-
dc.description.abstractIn a clinical trial of cerebral palsy, the level of plasma interleukin-8 (IL-8) was increased, correlated with motor improvement, after human umbilical cord blood mononuclear cell (hUCBC) infusion. This study aimed to elucidate the role of IL-8 in the therapeutic effects of hUCBCs in a mouse model of hypoxic-ischaemic brain injury (HI). In P7 HI mouse brains, hUCBC administration at day 7 after HI upregulated the gene expression of Cxcl2, the mouse IL-8 homologue and increased the expression of its receptor, CXCR2. hUCBC administration restored the sequential downstream signalling axis of p-p38/p-MAPKAPK2, NFκB, and angiogenic factors, which were downregulated by HI. An in vitro assay revealed the downregulation of the angiogenic pathway by CXCR2 knockdown and p38 inhibition. In vivo p38 inhibition prior to hUCBC administration in HI mouse brains produced identical results. Behavioural outcomes revealed a therapeutic effect (ps < 0.01) of hUCBC or IL-8 administration, which was correlated with decreases in infarct size and angiogenic findings in the striatum. In conclusion, the response of the host to hUCBC administration in mice upregulated Cxcl2, which led to the activation of the IL-8-mediated p-p38 signalling pathway. The upregulation of the downstream pathway and angiogenic growth factors via NFκB can be inferred to be the potential therapeutic mechanism of hUCBCs.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherNature Publishing Group-
dc.relation.isPartOfSCIENTIFIC REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleTherapeutic mechanism of cord blood mononuclear cells via the IL-8-mediated angiogenic pathway in neonatal hypoxic-ischaemic brain injury-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Physiology (생리학교실)-
dc.contributor.googleauthorKye Hee Cho-
dc.contributor.googleauthorJee In Choi-
dc.contributor.googleauthorJin-Ock Kim-
dc.contributor.googleauthorJoo Eun Jung-
dc.contributor.googleauthorDong-Wook Kim-
dc.contributor.googleauthorMinYoung Kim-
dc.identifier.doi10.1038/s41598-020-61441-0-
dc.contributor.localIdA00406-
dc.relation.journalcodeJ02646-
dc.identifier.eissn2045-2322-
dc.identifier.pmid32157146-
dc.contributor.alternativeNameKim, Dong Wook-
dc.contributor.affiliatedAuthor김동욱-
dc.citation.volume10-
dc.citation.number1-
dc.citation.startPage4446-
dc.identifier.bibliographicCitationSCIENTIFIC REPORTS, Vol.10(1) : 4446, 2020-03-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers

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