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Fabrication of high quality cDNA microarray using a small amount of cDNA

Authors
 Chan Hee Park  ;  Ha Jin Jeong  ;  Jae Jun Jung  ;  Gui Yeon Lee  ;  Sang-Chul Kim  ;  Tae Soo Kim  ;  Sang Hwa Yang  ;  Hyun Cheol Chung  ;  Sun Young Rha 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Vol.13(5) : 675-679, 2004-05 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 
ISSN
 1107-3756 
Issue Date
2004-05
MeSH
DNA, Complementary / analysis* ; DNA, Complementary / genetics* ; Dimethyl Sulfoxide / chemistry ; Glass ; Humidity ; Oligonucleotide Array Sequence Analysis / instrumentation* ; Oligonucleotide Array Sequence Analysis / methods* ; Quality Control* ; Reproducibility of Results ; Sensitivity and Specificity ; Silanes / chemistry ; Solutions / chemistry
Abstract
DNA microarray technology has become an essential part of biological research. It enables the genome-scale analysis of gene expression in various types of model systems. Manufacturing high quality cDNA microarrays of microdeposition type depends on some key factors including a printing device, spotting pins, glass slides, spotting solution, and humidity during spotting. UsingEthe Microgrid II TAS model printing device, this study defined the optimal conditions for producing high density, high quality cDNA microarrays with the least amount of cDNA product. It was observed that aminosilane-modified slides were superior to other types of surface modified-slides. A humidity of 30+/-3% in a closed environment and the overnight drying of the spotted slides gave the best conditions for arraying. In addition, the cDNA dissolved in 30% DMSO gave the optimal conditions for spotting compared to the 1X ArrayIt, 3X SSC and 50% DMSO. Lastly, cDNA in the concentration range of 100-300 ng/ micro l was determined to be best for arraying and post-processing. Currently, the printing system in this study yields reproducible 9000 spots with a spot size 150 mm diameter, and a 200 nm spot spacing.
Full Text
https://www.spandidos-publications.com/ijmm/13/5/675
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178874
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