Cited 12 times in
Tumor-specific gene therapy for uterine cervical cancer using MN/CA9-directed replication-competent adenovirus
DC Field | Value | Language |
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dc.contributor.author | 정현철 | - |
dc.date.accessioned | 2020-09-04T02:07:34Z | - |
dc.date.available | 2020-09-04T02:07:34Z | - |
dc.date.issued | 2004-08 | - |
dc.identifier.issn | 0929-1903 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/178825 | - |
dc.description.abstract | Although gene therapies using tissue-specific promoters have been reported to be a promising tool for treating cancers, few studies have explored this possibility for uterine cervical cancer. MN/CA9 is a transmembrane glycoprotein that was first identified in the human cervical carcinoma cell line, HeLa. Since MN/CA9 protein is highly expressed in uterine cervical cancer tissues, but not in normal cervix, we constructed a tumor-specific replication-competent adenoviral vector utilizing MN/CA9 promoter (Ad-MN/CA9-E1a), which can replicate only in MN/CA9-expressing cells. Infection of Ad-MN/CA9-E1a to MN/CA9-positive uterine cervical cancer cells (HeLa, C-33 A and SiHa) resulted in much stronger Ad5 E1a protein expressions compared with MN/CA9-negative cells (SK-RC-29), suggesting a tissue-specific replication of this recombinant adenovirus. In vitro cytotoxicity assay revealed that the growth of MN/CA9-positive cells was significantly inhibited with 0.01-1 MOI of Ad-MN/CA9-E1a, but the growth of MN/CA9-negative cells (SK-RC-29) could only be inhibited by as many as 100 MOI. Intratumoral injection of Ad-MN/CA9-E1a effectively induced growth delay of HeLa tumors in nude mice. These results suggest that a novel replication-competent adenoviral vector mediated by MN/CA9 promoter, Ad-MN/CA9-E1a, can selectively replicate in MN/CA9-expressing tumors with cytotoxic effects and may be utilized for the treatment of uterine cervical cancer. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | CANCER GENE THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Adenoviridae / genetics* | - |
dc.subject.MESH | Adenovirus E1A Proteins / biosynthesis | - |
dc.subject.MESH | Adenovirus E1A Proteins / genetics | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, Neoplasm / genetics* | - |
dc.subject.MESH | Antigens, Neoplasm / metabolism | - |
dc.subject.MESH | Carbonic Anhydrase IX | - |
dc.subject.MESH | Carbonic Anhydrases / genetics* | - |
dc.subject.MESH | Carbonic Anhydrases / metabolism | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Genetic Therapy / methods* | - |
dc.subject.MESH | Genetic Vectors / genetics | - |
dc.subject.MESH | HeLa Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Promoter Regions, Genetic / genetics | - |
dc.subject.MESH | RNA, Messenger / analysis | - |
dc.subject.MESH | RNA, Messenger / metabolism | - |
dc.subject.MESH | Uterine Cervical Neoplasms / genetics | - |
dc.subject.MESH | Uterine Cervical Neoplasms / metabolism | - |
dc.subject.MESH | Uterine Cervical Neoplasms / therapy* | - |
dc.subject.MESH | Virus Replication / genetics | - |
dc.subject.MESH | Xenograft Model Antitumor Assays | - |
dc.title | Tumor-specific gene therapy for uterine cervical cancer using MN/CA9-directed replication-competent adenovirus | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Ho Yeong Lim | - |
dc.contributor.googleauthor | Miwon Ahn | - |
dc.contributor.googleauthor | Hyun Cheol Chung | - |
dc.contributor.googleauthor | Thomas A Gardner | - |
dc.contributor.googleauthor | Chinghai Kao | - |
dc.contributor.googleauthor | Sang-Jin Lee | - |
dc.contributor.googleauthor | Se Joong Kim | - |
dc.identifier.doi | 10.1038/sj.cgt.7700732 | - |
dc.contributor.localId | A03773 | - |
dc.relation.journalcode | J00442 | - |
dc.identifier.eissn | 1476-5500 | - |
dc.identifier.pmid | 15167900 | - |
dc.identifier.url | https://www.nature.com/articles/7700732 | - |
dc.contributor.alternativeName | Chung, Hyun Cheol | - |
dc.contributor.affiliatedAuthor | 정현철 | - |
dc.citation.volume | 11 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 532 | - |
dc.citation.endPage | 538 | - |
dc.identifier.bibliographicCitation | CANCER GENE THERAPY, Vol.11(8) : 532-538, 2004-08 | - |
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