Cited 49 times in
The roles of sterol regulatory element-binding proteins in the transactivation of the rat ATP citrate-lyase promoter
DC Field | Value | Language |
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dc.contributor.author | 김경섭 | - |
dc.contributor.author | 박상욱 | - |
dc.date.accessioned | 2020-09-04T01:58:02Z | - |
dc.date.available | 2020-09-04T01:58:02Z | - |
dc.date.issued | 2000-09 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/178714 | - |
dc.description.abstract | ATP citrate-lyase (ACL) is a key enzyme supplying acetyl-CoA for fatty acid and cholesterol synthesis. Its expression is drastically up-regulated when an animal is fed a low fat, high carbohydrate diet after prolonged fasting. In this report, we describe the role of sterol regulatory element-binding proteins (SREBPs) in the transactivation of the rat ACL promoter. ACL promoter activity was markedly stimulated by the overexpression of SREBP-1a and, to a lesser extent, by SREBP-2 in Alexander human hepatoma cells. The promoter elements responsive to SREBPs were located within the 55-base pair sequences from -114 to -60. The gel mobility shift assay revealed four SREBP-1a binding sites in this region. Of these four elements, the -102/-94 region, immediately upstream of the inverted Y-box, and the -70/-61 region, just adjacent to Sp1 binding site, played critical roles in SREBPs-mediated stimulation. The mutation in the inverted Y-box and the coexpression of dominant negative nuclear factor-Y (NF-Y) significantly attenuated the transactivation by SREBP-1a, suggesting that NF-Y binding is a prerequisite for SREBPs to activate the ACL promoter. However, the multiple Sp1 binding sites did not affect the transactivation of the ACL promoter by SREBPs. The binding affinity of SREBP-1a to SREs of the ACL promoter also was much higher than that of SREBP-2. The transactivation potencies of the chimeric SREBPs, of which the activation domains (70 amino acids of the amino terminus) were derived from the different species of their carboxyl-terminal region, were similar to those of SREBPs corresponding to their carboxyl termini. Therefore, it is suggested that the carboxyl-terminal portions of SREBPs containing DNA binding domains are important in determining their transactivation potencies to a certain promoter. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | ATP Citrate (pro-S)-Lyase / genetics* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Binding Sites / genetics | - |
dc.subject.MESH | CCAAT-Binding Factor / metabolism | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Proteins / metabolism* | - |
dc.subject.MESH | DNA Footprinting | - |
dc.subject.MESH | DNA-Binding Proteins / metabolism* | - |
dc.subject.MESH | Deoxyribonuclease I | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Liver / enzymology | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Protein Binding | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Sterol Regulatory Element Binding Protein 1 | - |
dc.subject.MESH | Sterol Regulatory Element Binding Protein 2 | - |
dc.subject.MESH | Transcription Factors / metabolism* | - |
dc.subject.MESH | Transcriptional Activation* | - |
dc.title | The roles of sterol regulatory element-binding proteins in the transactivation of the rat ATP citrate-lyase promoter | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.googleauthor | Y A Moon | - |
dc.contributor.googleauthor | J J Lee | - |
dc.contributor.googleauthor | S W Park | - |
dc.contributor.googleauthor | Y H Ahn | - |
dc.contributor.googleauthor | K S Kim | - |
dc.identifier.doi | 10.1074/jbc.M001066200 | - |
dc.contributor.localId | A00297 | - |
dc.contributor.localId | A01487 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 10801800 | - |
dc.contributor.alternativeName | Kim, Kyung Sup | - |
dc.contributor.affiliatedAuthor | 김경섭 | - |
dc.contributor.affiliatedAuthor | 박상욱 | - |
dc.citation.volume | 275 | - |
dc.citation.number | 39 | - |
dc.citation.startPage | 30280 | - |
dc.citation.endPage | 30286 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.275(39) : 30280-30286, 2000-09 | - |
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