Cited 7 times in
Generation of Functional Cardiomyocytes from the Synoviocytes of Patients with Rheumatoid Arthritis via Induced Pluripotent Stem Cells
DC Field | Value | Language |
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dc.contributor.author | 정승민 | - |
dc.date.accessioned | 2020-07-27T16:46:22Z | - |
dc.date.available | 2020-07-27T16:46:22Z | - |
dc.date.issued | 2016-09 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/178519 | - |
dc.description.abstract | Cardiovascular disease is a leading cause of morbidity in rheumatoid arthritis (RA) patients. This study aimed to generate and characterise cardiomyocytes from induced pluripotent stem cells (iPSCs) of RA patients. Fibroblast-like synoviocytes (FLSs) from patients with RA and osteoarthritis (OA) were successfully reprogrammed into RA-iPSCs and OA-iPSCs, respectively. The pluripotency of iPSCs was confirmed by quantitative reverse transcription-polymerase chain reaction and immunofluorescence staining. Established iPSCs were differentiated into cardiomyocytes using a small molecule-based monolayer differentiation protocol. Within 12 days of cardiac differentiation from patient-specific and control-iPSCs, spontaneously beating cardiomyocytes (iPSC-CMs) were observed. All iPSC-CMs exhibited a reliable sarcomeric structure stained with antibodies against cardiac markers and similar expression profiles of cardiac-specific genes. Intracellular calcium signalling was recorded to compare calcium-handling properties among cardiomyocytes differentiated from the three groups of iPSCs. RA-iPSC-CMs had a lower amplitude and a shorter duration of calcium transients than the control groups. Peak tangential stress and the maximum contractile rate were also decreased in RA-iPSC-CMs, suggesting that contractility was reduced. This study demonstrates the successful generation of functional cardiomyocytes from pathogenic synovial cells in RA patients through iPSC reprogramming. Research using RA-iPSC-CMs might provide an opportunity to investigate the pathophysiology of cardiac involvement in RA. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Arthritis, Rheumatoid / pathology* | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Induced Pluripotent Stem Cells / pathology* | - |
dc.subject.MESH | Myocytes, Cardiac / pathology* | - |
dc.subject.MESH | Synoviocytes / pathology* | - |
dc.title | Generation of Functional Cardiomyocytes from the Synoviocytes of Patients with Rheumatoid Arthritis via Induced Pluripotent Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Jaecheol Lee | - |
dc.contributor.googleauthor | Seung Min Jung | - |
dc.contributor.googleauthor | Antje D Ebert | - |
dc.contributor.googleauthor | Haodi Wu | - |
dc.contributor.googleauthor | Sebastian Diecke | - |
dc.contributor.googleauthor | Youngkyun Kim | - |
dc.contributor.googleauthor | Hyoju Yi | - |
dc.contributor.googleauthor | Sung-Hwan Park | - |
dc.contributor.googleauthor | Ji Hyeon Ju | - |
dc.identifier.doi | 10.1038/srep32669 | - |
dc.contributor.localId | A05179 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 27609119 | - |
dc.contributor.alternativeName | Jung, SeungMin | - |
dc.contributor.affiliatedAuthor | 정승민 | - |
dc.citation.volume | 6 | - |
dc.citation.startPage | 32669 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.6 : 32669, 2016-09 | - |
dc.identifier.rimsid | 64801 | - |
dc.type.rims | ART | - |
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