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Pectinase-treated Panax ginseng ameliorates hydrogen peroxide-induced oxidative stress in GC-2 sperm cells and modulates testicular gene expression in aged rats
DC Field | Value | Language |
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dc.contributor.author | 서석교 | - |
dc.date.accessioned | 2020-07-27T16:43:37Z | - |
dc.date.available | 2020-07-27T16:43:37Z | - |
dc.date.issued | 2016-04 | - |
dc.identifier.issn | 1226-8453 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/178487 | - |
dc.description.abstract | Background: To investigate the effect of pectinase-treated Panax ginseng (GINST) in cellular and male subfertility animal models. Methods: Hydrogen peroxide (H2O2)-induced mouse spermatocyte GC-2spd cells were used as an in vitro model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein expression level of antioxidative and spermatogenic genes in young and aged control rats were compared using real-time reverse transcription polymerase chain reaction and western blotting. Results: GINST treatment (50 μg/mL, 100 μg/mL, and 200 μg/mL) significantly (p < 0.05) inhibited the H2O2-induced (200 μM) cytotoxicity in GC-2spd cells. Furthermore, GINST (50 μg/mL and 100 μg/mL) significantly (p < 0.05) ameliorated the H2O2-induced decrease in the expression level of antioxidant enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4), spermatogenesis-related protein such as inhibin-α, and specific sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells. Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST (200 mg/kg) treatment. Taken together, GINST attenuated H2O2-induced oxidative stress in GC-2 cells and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and sex hormone receptors in aged rats. Conclusion: GINST may be a potential natural agent for the protection against or treatment of oxidative stress-induced male subfertility and aging-induced male subfertility. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English, Korean | - |
dc.publisher | Korean Society of Ginseng | - |
dc.relation.isPartOf | JOURNAL OF GINSENG RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Pectinase-treated Panax ginseng ameliorates hydrogen peroxide-induced oxidative stress in GC-2 sperm cells and modulates testicular gene expression in aged rats | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Obstetrics and Gynecology (산부인과학교실) | - |
dc.contributor.googleauthor | Spandana Rajendra Kopalli | - |
dc.contributor.googleauthor | Kyu-Min Cha | - |
dc.contributor.googleauthor | Min-Sik Jeong | - |
dc.contributor.googleauthor | Sang-Ho Lee | - |
dc.contributor.googleauthor | Jong-Hwan Sung | - |
dc.contributor.googleauthor | Seok-Kyo Seo | - |
dc.contributor.googleauthor | Si-Kwan Kim | - |
dc.identifier.doi | 10.1016/j.jgr.2015.08.005 | - |
dc.contributor.localId | A01888 | - |
dc.relation.journalcode | J01424 | - |
dc.identifier.eissn | 2093-4947 | - |
dc.identifier.pmid | 27158240 | - |
dc.subject.keyword | Panax ginseng | - |
dc.subject.keyword | oxidative enzymes | - |
dc.subject.keyword | pectinase | - |
dc.subject.keyword | spermatogenesis | - |
dc.subject.keyword | subfertility | - |
dc.contributor.alternativeName | Seo, Seok Kyo | - |
dc.contributor.affiliatedAuthor | 서석교 | - |
dc.citation.volume | 40 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 185 | - |
dc.citation.endPage | 195 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GINSENG RESEARCH, Vol.40(2) : 185-195, 2016-04 | - |
dc.identifier.rimsid | 64822 | - |
dc.type.rims | ART | - |
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