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Retroviral Vector를 이용한 인체 간암세포주로의 Interleukin-2 유전자 형질도입의 효과
DC Field | Value | Language |
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dc.contributor.author | 김주항 | - |
dc.contributor.author | 노재경 | - |
dc.contributor.author | 라선영 | - |
dc.date.accessioned | 2020-07-03T17:42:02Z | - |
dc.date.available | 2020-07-03T17:42:02Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 0496-6872 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/177769 | - |
dc.description.abstract | PURPOSE: We compared the differences between parent hepatoma cell lines and interleukin-2 (IL-2) transduced hepatoma cell lines using N2A/IL-2 and LNC/IL-2 retrovirus with regards to in vitro sensitivity to peripheral blood monocytes and in vivo tumorigenic activity. MATERIALS AND METHODS: Retroviral vector and producer cell line were constructed and IL-2 gene was transduced into the human hepatoma cell lines (SK-Hep1, Hep-G2, Hep-3B). IL-2 secretion after IL-2 transduction was measured by ELISA. MTT assay for in vitro sensitivity to peripheral blood monocytes was performed and the tumorigenic activity was observed in BALB/c mice and nude mice. RESULTS: IL-2 secretion was 186 pg/10 degrees C cells/24 hrs in SK-Hep1 cell line and was 147 pg/10 (6) cells/24 hrs in Hep-3B cell line with N2A/IL-2 retroviral vector and was 55,000 pg/10 (6) cells/24 hrs with LNC/IL-2 retroviral vector. In vitro sensitivity to peripheral blood monocytes was increased by 163.8~254% in IL-2 transduced hepatoma cell lines (Hep -3B/N2A/IL-2, Hep-G2/N2A/IL-2) compared to those of the parent cell lines. The tumorigenicity was observed in 1 of 3 BALB/c mice and all 3 nude mice. Simultaneous injection of 1 X 10 (7) cells of the parent cell line (Hep-3B) into the right flank and IL-2 transduced cell line (Hep-3B/LNC/IL-2) into the left flank of the three BALB/c mice and of 5 X 10 (5) cells for the three nude mice resulted in a complete regression of the IL-2 modified tumor cell line (Hep-3B/LNC/IL-2) in 3 weeks and the parent cell line (Hep-3B) in 5 weeks. But, after the injection of 1.5 X 10 (7) cells for other five nude mice, the tumor of the IL-2 transduced hepatoma cell line (Hep-3B/LNC/IL-2) was gradually disappeared, and the tumor of the parent hepatoma cell line (Hep-3B) was initially decreased and then gradually regrew 20 days later. CONCLUSION: IL-2 transduced hepatoma cell lines secreting IL-2 became more sensitive to peripheral blood monocytes and resulted in the increased antigenicity to the tumors formed by IL-2 transduced hepatoma cell line and parent cell line, and finally resulted in the regression of the tumors in experimental animals. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 대한암학회 | - |
dc.relation.isPartOf | Journal of the Korean Cancer Association (대한암학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Retroviral Vector를 이용한 인체 간암세포주로의 Interleukin-2 유전자 형질도입의 효과 | - |
dc.title.alternative | Effects of Interleukin-2 Transduction into the Human Hepatoma Cell Lines Using Retroviral Vector | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | 공수정 | - |
dc.contributor.googleauthor | 유내춘 | - |
dc.contributor.googleauthor | 김주황 | - |
dc.contributor.googleauthor | 신동환 | - |
dc.contributor.googleauthor | 엄효동 | - |
dc.contributor.googleauthor | 정숙정 | - |
dc.contributor.googleauthor | 조재용 | - |
dc.contributor.googleauthor | 라선영 | - |
dc.contributor.googleauthor | 김연수 | - |
dc.contributor.googleauthor | 정현철 | - |
dc.contributor.googleauthor | 노재경 | - |
dc.contributor.googleauthor | 민진식 | - |
dc.contributor.googleauthor | 김병수 | - |
dc.contributor.localId | A00945 | - |
dc.contributor.localId | A01290 | - |
dc.contributor.localId | A01316 | - |
dc.relation.journalcode | J01813 | - |
dc.subject.keyword | Interleukin-2 | - |
dc.subject.keyword | Hepatoma cell line | - |
dc.subject.keyword | Tumor regression | - |
dc.contributor.alternativeName | Kim, Joo Hang | - |
dc.contributor.affiliatedAuthor | 김주항 | - |
dc.contributor.affiliatedAuthor | 노재경 | - |
dc.contributor.affiliatedAuthor | 라선영 | - |
dc.citation.volume | 29 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 555 | - |
dc.citation.endPage | 564 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Cancer Association (대한암학회지), Vol.29(4) : 555-564, 1997 | - |
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