성분채집혈소판에서의 혈소판 활성화 및 저장 중 발생하는 손상의 평가

Abstract

Background : Three cell separators are being used and they collect platelets with different centrifuge speed and duration. Because centrifugation may cause platelet activation, the differences of centrifuge speed and duration are important in controlling the quality of apheresis platelet products. We compared many parameters of activation of platelets collected by Spectra (Cobe BCT, Lakewood, CO, USA), CS3000p1us (Banter Healthcare, Fenwal Division, Round Lake, IL, USA) and Mobile Collection SystemTM (MCS, Haemonetics co., Braintree, MA, USA). Methods : Platelets were collected from ninety-five normal donors with Spectra (n=39) CS3000p1us (n=19) and MCS (n=37). We underwent the procedure according to the automatic program set. We measured platelet yield and assayed pH, hypotonic shock respose (HSR), CD62 (p-selectin, GMP140) expression and β-thromboglobulin in each stored unit on day 0 and day 3 (or evaluation of the storage lesions. Results : Platelet yield per product was 3.7±1.2×1011, mean final product volume was 316±69mL and mean procession time was 100±19 minutes. Mean collection efficiency was 42.5±8.3%. The cell separator volume of product collected by CS3000p1us was the smallest while platelet concentration and total yield were the highest in the product collected by Spectra. The pH of the products were 7.1±0.1 on day 0 and 6.7± 0.4 on day 3 Hypotonic shock response was 69±13 % on day 0 and 28 ±17 % on day 3. p-selectin expression was 19±9% (4.2±1.9 relative fluorescence intensity, RFI) on day 0 and 60±22% (17.9±14.2) on day 3. β-thromboglobulin was 28.5±7.0 IU/107 platelets on day 0 and 31.3±7.2 IU/107 platelets on day 3. The comparison of the three cell separators showed that on day 0 platelet product of MCS has lower pH and higher β-thromboglobulin release than others (p<0.05). And on day 3 platelet product of MDS has better hypotonic shock response than others (p<0.05) Other parameters revealed no differences among three cell separators. The expression of p-selectin was shown to correlate highly with pH reduction (r=0.72), but not with the release of β-7G (r=0.24). Conclusions : Most parameters showed no differences among three cell separators, but apheresis platelet concentrates processed by MCS showed lower pH on day 0 and higher β-thromboglobulin concentration on day 0 and day 3 than apheresis platelet concentrates processed by Spectra or CS3000p1us and hypotonic shock response on day 3 was the lowest in CS3000p1us. So platelet activation produced during apheresis processing was lowest in apheresis platelet concentrates with Spectra.