Cited 0 times in
간세포암종에 동반된 거대 재생결절의 클론성에 관한 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김호근 | - |
dc.date.accessioned | 2020-07-03T17:31:15Z | - |
dc.date.available | 2020-07-03T17:31:15Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 0379-1149 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/177614 | - |
dc.description.abstract | In order to clarify the preneoplastic nature of large regenerative nodules without dysplastic change, we analysed the clonality of hepatocellular carcinomas (HCCs) and large nodules, diameter > or =0.5 cm, of cirrhotic liver by X-linked human androgen receptor (HUMARA) gene assay, using the principle of random X chromosome methylation and inactivation in female. Ten cases of HCC and 5 cases of large nodules without dysplasia from 9 female patients were selected. All the tumors, large nodules and paired normal control cells were selectively microdissected from deparaffinized hematoxylin and eosin stained slides. Genomic DNA was isolated and digested with HhaI. Polymerase chain reaction(PCR) amplication of the HUMARA locus was performed using 32P-a-dCTP containing PCR mixtures. The PCR amplified products were separated by gel electrophoresis and analysed by autoradiography. Nine HCCs from 8 patients were monoclonal and 1 case was polyclonal and the remaining 1 case was not polymorphic at the HUMARA locus. The HCC case which showed polyclonality contained many inflammatory cells. All the large nodules were polyclonal by HUMARA assay. These results suggest that all or most of the cells composing the large regenerative nodules without dysplasia are polyclonal. This assay may be informative for the differentiation between regenerative and preneoplastic nodules in cirrhotic liver and the size of nodule may be not important in hepatocarcinogenesis. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 대한병리학회 | - |
dc.relation.isPartOf | Korean Journal of Pathology (대한병리학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | 간세포암종에 동반된 거대 재생결절의 클론성에 관한 연구 | - |
dc.title.alternative | Clonality of Large Regenerative Nodule Accompanied by Hepatocellular Carcinoma | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학교실) | - |
dc.contributor.googleauthor | 박철 | - |
dc.contributor.googleauthor | 전봉균 | - |
dc.contributor.googleauthor | 이우정 | - |
dc.contributor.googleauthor | 박영년 | - |
dc.contributor.googleauthor | 김호근 | - |
dc.contributor.googleauthor | 박찬일 | - |
dc.contributor.localId | A01183 | - |
dc.relation.journalcode | J02093 | - |
dc.contributor.alternativeName | Kim, Hogeun | - |
dc.contributor.affiliatedAuthor | 김호근 | - |
dc.citation.volume | 31 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 884 | - |
dc.citation.endPage | 890 | - |
dc.identifier.bibliographicCitation | Korean Journal of Pathology (대한병리학회지), Vol.31(9) : 884-890, 1997 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.