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Induction of Apoptosis and Expression of Apoptosis-related Gene Products in Response to Radiation in Murine Tumors
DC Field | Value | Language |
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dc.contributor.author | 성진실 | - |
dc.date.accessioned | 2020-07-03T17:12:32Z | - |
dc.date.available | 2020-07-03T17:12:32Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 1229-8719 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/177348 | - |
dc.description.abstract | PURPOSE: To analyze the involvement of apoptosis regulatory genes p53, p21waf1/cip1, bax and bcl-2 in induction of apoptosis by radiation in murine tumors. MATERIALS AND METHODS: The radiation-sensitive ovarian carcinoma OCa-I, and the radiation-resistant hepatocarcinoma HCa-I were used. Tumors, 8 mm in diameter, were irradiated with 25 Gy and at various times after irradiation, ranging from 1 to 48 h, were analyzed histologically for apoptosis and by western blot for alterations in the expression of these genes. The p53 status of the tumors were determined by the polymerase chain reaction-single strand conformation polymorphism assay. RESULTS: Both tumors were positive for wild-type p53. Radiation induced apoptosis in OCa-I but not in HCa-I. Apoptosis developed rapidly, peaked at 2 h after irradiation and returned to almost the background level at 48 h. In OCa-I radiation upregulated the expression of p53, p21waf1/cip1, and the bcl-2/bax ratio was decreased. In HCa-I radiation increased the expression of both p53 and p21waf1/cip1, although the increase of the latter was small. The bcl-2/bax ratio was greatly increased. In general the observed changes occurred within a few hours after irradiation, and either preceded or coincided with development of apoptosis. CONCLUSIONS: The development of apoptosis required upregulation of both p53 and p21waf1/cip1 as well as a decrease in bcl-2/bax ratio. In contrast, an increase in bcl-2/bax ratio prevented apoptosis in the presence of upregulated p53 and p21waf1/cip1. These findings indentified the involvement of multiple oncogenes in apoptosis regulation in vivo and demonstrate the complexity that may be associated with the use of a single oncogene assessment for predicting the outcome of cancer therapy with cytotoxic agents. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 대한방사선종양학회 | - |
dc.relation.isPartOf | Journal of the Korean Society for Therapeutic Radiology and Oncology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Induction of Apoptosis and Expression of Apoptosis-related Gene Products in Response to Radiation in Murine Tumors | - |
dc.title.alternative | 방사선에 대한 종양의 반응에서 아포프토시스의 유도와 이에 관련되는 유전자 발현 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학교실) | - |
dc.contributor.googleauthor | Jinsil Seong | - |
dc.contributor.googleauthor | Nancy R. Hunter | - |
dc.contributor.googleauthor | Luka Milas | - |
dc.contributor.localId | A01956 | - |
dc.relation.journalcode | J01857 | - |
dc.contributor.alternativeName | Seong, Jin Sil | - |
dc.contributor.affiliatedAuthor | 성진실 | - |
dc.citation.volume | 15 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 187 | - |
dc.citation.endPage | 195 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Society for Therapeutic Radiology and Oncology, Vol.15(3) : 187-195, 1997 | - |
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