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거대세포바이러스 감염증의 조기진단을 위한 PCR및 early antigen 면역염색법의 유용

DC FieldValueLanguage
dc.contributor.author김현숙-
dc.date.accessioned2020-07-02T17:51:28Z-
dc.date.available2020-07-02T17:51:28Z-
dc.date.issued1998-
dc.identifier.issn1015-6445-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/177079-
dc.description.abstractBackground :Cytomegalovirus infection is an important cause of morbidity and mortality alter organ transplantation. Thus, rapid, sensitive and specific laboratory test, such as CMV anti-genemia assay and polymerase chain reaction (PCR) is necessary to determine a patient`s risk of CMV disease and to monitor the effectiveness of antiviral therapy. We compared the results of CMV-PCR and CMV early antigen immunostaining (CMV-EA) with CMV-specific IgM antibody to evalutate clinical usefulness for the early diagnosis of CMV infection anti monitoring of antiviral therapy Method :We analyzed 170 samples submitted for CMV tests between September 1995 and April 1996 in Yonsei University College of Medicine Severance Hospital. CMV-PCR and CMV-EA were performed with buffy coat cells and detection of CMV-specific IgM antibody was performed by enzyme-linked fluorescent assay (ELFA) Result :One hundred and seventy samples of 159 patients were tested anti analyzed The concordance rate of CMV-PCR, CMV-EA anti CMV-specific IgM In the same blood sample was 753%. The total Incidence of CMV disease was 25%. The sensitivity anti specificity based on the patients clinical status of PCR were 100% and 91.6% respectively in CMV-EA immunostaining method, they were 750% and 100% respectively, And, for CMV-specific IgM antibody ELFA, the sensitivity was only 500% anti the specificity was 96.4%. Conclusion :CMV-PCR and CMV-EA immunostaining are reliable methods as rapid early detection of CMV infection. The sensitivity and specificity are very high comparing to CMV-specific IgM antibody. It could also be concluded that they haute advantages not only for early diagnosis but also monitoring or follow-up of a therapeutic course as quantitative assays.-
dc.description.statementOfResponsibilityopen-
dc.languageKorean-
dc.publisher대한임상병리학회-
dc.relation.isPartOfKorean Journal of Clinical Pathology (대한임상병리학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.title거대세포바이러스 감염증의 조기진단을 위한 PCR및 early antigen 면역염색법의 유용-
dc.title.alternativeThe Usefulness of PCR and Early Antigen Immunostaining as a Rapid Identification Method of Cytomegalovirus Infection-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.googleauthor신혜정-
dc.contributor.googleauthor김현경-
dc.contributor.googleauthor김현숙-
dc.contributor.localIdA01117-
dc.relation.journalcodeJ01988-
dc.contributor.alternativeNameKim, Hyon Suk-
dc.contributor.affiliatedAuthor김현숙-
dc.citation.volume18-
dc.citation.number3-
dc.citation.startPage452-
dc.citation.endPage457-
dc.identifier.bibliographicCitationKorean Journal of Clinical Pathology (대한임상병리학회지), Vol.18(3) : 452-457, 1998-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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