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Hyperoxia influences mRNA expression of cytokines in cultured human umbilical vein endothelial cells

Authors
 Min Soo Park  ;  Heather M Wallace 
Citation
 YONSEI MEDICAL JOURNAL, Vol.39(1) : 1-12, 1998 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
1998
MeSH
Cells, Cultured ; Cytokines/genetics* ; DNA/biosynthesis ; Endothelium, Vascular/metabolism* ; Humans ; Hyperoxia/metabolism* ; RNA, Messenger/analysis* ; Umbilical Veins
Abstract
High concentrations of oxygen, indispensable for the treatment of severe hypoxemia from neonatal as well as adult respiratory distress syndrome, increase the risk of oxygen toxicity. Biochemical mechanisms are lipid peroxidation, protein sulfhydryl oxidation, enzyme inactivation, and DNA damage. Recent reports suggest that cytokines might be involved in free radical injury as well as in adaptive response to hyperoxic injury. However, actual signal transduction pathways involving cytokines have not yet been clarified. In this study we exposed cultured human umbilical vein endothelial cells (HUVECs) to either ambient air or 100% oxygen, and compared for the rate of DNA synthesis ([3H]thymidine uptake) at different time points up to 72 h. After exposing the cells to each treatment condition, we extracted RNA, constructed complementary DNA using reverse transcriptase, amplified the specific DNA segments of cytokines by polymerase chain reaction (PCR), and used the PCR products for gel electrophoresis to examine the bands which signified mRNA levels of corresponding cytokines. There was a significant decrease in the rate of DNA synthesis as early as 24 h. The mRNA expression of IL-1 β and TNFa seemed less influenced by hyperoxia, while IL-8 and TGF β showed marked increase in mRNA levels at 6 h of 100% oxygen exposure.
Files in This Item:
T199802381.pdf Download
DOI
10.3349/ymj.1998.39.1.1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Park, Min Soo(박민수) ORCID logo https://orcid.org/0000-0002-4395-9938
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/176812
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