Cited 35 times in
Extensive analysis of duplicated-inverted hepatitis B virus integrations in human hepatocellular carcinoma
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김원호 | - |
dc.date.accessioned | 2020-07-02T17:17:02Z | - |
dc.date.available | 2020-07-02T17:17:02Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0022-1317 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176716 | - |
dc.description.abstract | Hepatitis B virus (HBV) DNA is found chromosomally integrated into the genome of the majority of hepatocellular carcinomas (HCC) arising in chronic HBV carriers suggesting that, in some instances, viral sequences may be directly responsible for oncogenic conversion. In an attempt to clarify the oncogenic potential of integrated HBV sequences, we performed an extensive analysis of two single integrations present in HCC which developed in non-cirrhotic livers from HBsAg-positive Korean patients. In both cases, integrated viral sequences were characterized by a duplicated-inverted configuration involving the flanking cellular sequences, a pattern consistently found in many amplicons isolated from mammalian cells. Integration sites are characterized by an AT-rich content and the presence of topoisomerase I and II cleavage target sequences as well as other recombination-prone motifs. The chromosomal locations of the integration sites were determined as 8q13 and 10q22 in the human genome, two regions known to harbour genes involved in tumorigenesis. The cis-activating potential of the integrations in their original configuration was also investigated in a transient transfection assay in HepG2 cells. Integrated sequences, rather than activating heterologous promoters, show either no activity or a weak tendency to inhibit activation of neighbouring reporter genes. The implications of our findings for the understanding of primary liver cancer development are discussed. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Microbiology Society | - |
dc.relation.isPartOf | JOURNAL OF GENERAL VIROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Carcinoma, Hepatocellular/virology* | - |
dc.subject.MESH | Chromosomes, Human, Pair 10 | - |
dc.subject.MESH | Chromosomes, Human, Pair 8 | - |
dc.subject.MESH | DNA, Viral/analysis | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Hepatitis B virus/genetics* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | In Situ Hybridization, Fluorescence | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Recombination, Genetic | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.subject.MESH | Virus Integration* | - |
dc.title | Extensive analysis of duplicated-inverted hepatitis B virus integrations in human hepatocellular carcinoma | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Pascal Pineau | - |
dc.contributor.googleauthor | Agne' s Marchio | - |
dc.contributor.googleauthor | Marie-Genevie' ve Mattei | - |
dc.contributor.googleauthor | Won-Ho Kim | - |
dc.contributor.googleauthor | Jung-Koo Youn | - |
dc.contributor.googleauthor | Pierre Tiollais | - |
dc.contributor.googleauthor | Anne Dejean | - |
dc.identifier.doi | 10.1099/0022-1317-79-3-591 | - |
dc.contributor.localId | A00774 | - |
dc.relation.journalcode | J01421 | - |
dc.identifier.eissn | 1465-2099 | - |
dc.identifier.pmid | 9519839 | - |
dc.identifier.url | https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-3-591#tab2 | - |
dc.contributor.alternativeName | Kim, Won Ho | - |
dc.contributor.affiliatedAuthor | 김원호 | - |
dc.citation.volume | 79 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 591 | - |
dc.citation.endPage | 600 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GENERAL VIROLOGY, Vol.79(3) : 591-600, 1998 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.