Cited 8 times in
Ex vivo generation of functional dendritic cells from mobilized CD34+ hematopoietic stem cells
DC Field | Value | Language |
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dc.contributor.author | 김현옥 | - |
dc.contributor.author | 민유홍 | - |
dc.date.accessioned | 2020-07-02T17:16:37Z | - |
dc.date.available | 2020-07-02T17:16:37Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176711 | - |
dc.description.abstract | The ability to generate dendritic cells (DCs) in sizeable numbers has enormous implications for the development of clinically-effective antigen presentation procedures for cancer immunotherapy. We evaluated the generation of immunostimulatory DCs from peripheral blood CD34+ cells collected from healthy donors. CD34+ cells purified from leukapheresis product were seeded at 1 x 104 cells/mL in complete medium supplemented with GM-CSF, TNF alpha, IL-4, c-kit ligand, and flt3 ligand (FL). By day 14 of culture in the presence of GM-CSF + TNF alpha, the total cell number increased by 23.4 ± 5.4-fold compared to the starting number of CD34+ cells. When the c-kit and FL were added to GM-CSF and TNF alpha, the cell number increased by 109.8 ± 11.2-fold without affecting the immunophenotype of recovered cells. Flow cytometric analysis indicated that cells with the markers of mature dendritic cells, i.e., CD1a +CD14 -HLA-DR+, and CD80+CD86+HLA-DR+, constituted 49.0% ± 7.5%, and 38.9% ± 6.5%, respectively. This pattern of expression of surface antigen was unchanged whether the c-kit ligand and/or FL was added. The irradiated CD1a+HLA-DR+ cells recovered from in vitro cultures elicit a vigorous proliferation of allogeneic peripheral blood T-cells, irrespective of cytokine combinations. These findings provide advantageous tools for the large-scale generation of DCs that are potentially usable for clinical protocols of immunotherapy or vaccination in patients undergoing cancer treatment. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Ex vivo generation of functional dendritic cells from mobilized CD34+ hematopoietic stem cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Yoo Hong Min | - |
dc.contributor.googleauthor | Seung Tae Lee | - |
dc.contributor.googleauthor | Kyung Mi Choi | - |
dc.contributor.googleauthor | So Young Chong | - |
dc.contributor.googleauthor | Hyun Ok Kim | - |
dc.contributor.googleauthor | Jee Sook Hahn | - |
dc.contributor.googleauthor | Yun Woong Ko | - |
dc.identifier.doi | 10.3349/ymj.1998.39.4.328 | - |
dc.contributor.localId | A01122 | - |
dc.contributor.localId | A01407 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 9752799 | - |
dc.contributor.alternativeName | Kim, Hyun Ok | - |
dc.contributor.affiliatedAuthor | 김현옥 | - |
dc.contributor.affiliatedAuthor | 민유홍 | - |
dc.citation.volume | 39 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 328 | - |
dc.citation.endPage | 338 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.39(4) : 328-338, 1998 | - |
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