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Immunodiagnosis of clonorchiasis using a recombinant antigen.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박순정 | - |
dc.contributor.author | 용태순 | - |
dc.date.accessioned | 2020-07-02T17:06:22Z | - |
dc.date.available | 2020-07-02T17:06:22Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176593 | - |
dc.description.abstract | A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a β-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis, C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English, Korean | - |
dc.publisher | Korean Society for Parasitology | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Immunodiagnosis of clonorchiasis using a recombinant antigen. | - |
dc.title.alternative | 간흡충 재조합항원을 이용한 간흡충증의 면역 진단 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Tropica Medicine (열대의학교실) | - |
dc.contributor.googleauthor | 용태순 | - |
dc.contributor.googleauthor | 양혜진 | - |
dc.contributor.googleauthor | 박순정 | - |
dc.contributor.googleauthor | 김유경 | - |
dc.contributor.googleauthor | 이두호 | - |
dc.contributor.googleauthor | 이상미 | - |
dc.contributor.localId | A01545 | - |
dc.contributor.localId | A02424 | - |
dc.relation.journalcode | J01957 | - |
dc.identifier.eissn | 1738-0006 | - |
dc.contributor.alternativeName | Park, Soon Jung | - |
dc.contributor.affiliatedAuthor | 박순정 | - |
dc.contributor.affiliatedAuthor | 용태순 | - |
dc.citation.volume | 36 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 183 | - |
dc.citation.endPage | 189 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PARASITOLOGY, Vol.36(3) : 183-189, 1998 | - |
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