Helicobacter pylori 감염과 세포증식 및 세포사멸간의 관계

Abstract

Background/Aims: Many studies have shown that Helicobacter pylori (H. pylori) infection is associated with gastroduodenal diseases. The pathogenic effects of H. pylori are associated with cytokine production, cell cycle progression, cellular proliferation and apoptosis. We performed this study to investigate the effect of H. pylori eradication on gastric mucosal cell apoptosis and proliferation. Methods: Gastroduodenoscopy was performed for 42 cases. Twenty two of them showed H. pylori-positive gastritis and the other 20 cases showed H. pylori-negative normal mucosa. H. pylori infection was assessed by rapid urease test and histology of biopsy specimens from gastric antrum. Ki-67 immunostaining of gastric biopsy specimens was used to measure mucosal cell proliferation in H. pylori positive gastritis before and after therapy. DNA frapnentation with terminal deoxynu- cleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling method was used for a marker of apoptosis. Results: Apoptosis was increased in gastric mucosal epithelium of patients with H. pylori-positive gastritis (p<0.05). H. pylori eradication therapy reduced apoptosis in antral mucosa (p<0.05) and the cell proliferation levels (p=0.051). Conclusions: Induction of apoptosis in gastric epithelium by chronic stimulation of H. pylori could be involved in the pathogenesis of gastroduodenal diseases.