Midazolam의 절제한 기니픽 기관평활근 이완에 미치는 Flumazenil 및 Verapamil의 영향

Abstract

Background: Midazolam relaxes airway smooth muscle. The aim of this study is to evaluate the influence of flumazenil or verapamil on the relaxation effects of midazolam in tracheal smooth muscle of guinea pig.

Methods: After isolating guinea-pig tracheal preparations, the maximal tracheal tones were induced by 2 X 10-7 M carbachol. When tracheal tones stabilized, midazolam was added cumulatively(10-6, 3 X 10-6, 10-5, 3 X 10-5, 10-4 M, n=14) with or without flumazenil(10-6 M, n=15) and verapamil(10-5 M, n=13) to obtain the concentration-relaxation curves, and then the ED50 and ED95 calculated.

Results: Midazolam decreased maximal tracheal smooth muscle tones in concentration-dependent manners. Pretreatment with flumazenil had no effect on the midazolam-induced relaxation. Verapamil enhanced the relaxation effect of midazolam.

Conclusions: Midazolam relaxes airway smooth muscle and has synergistic effect with calcium channel blocker, verapamil.