323 277

Cited 0 times in

진주종상피에서 Apoptosis를 통한 세포의 사멸기전

DC Field Value Language
dc.contributor.author정명현-
dc.date.accessioned2020-07-02T16:56:36Z-
dc.date.available2020-07-02T16:56:36Z-
dc.date.issued1998-
dc.identifier.issn1225-035X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/176472-
dc.description.abstractBackground and Objectives:Accumulation of keratin debris in the middle ear is one of the characteristics of the cholesteatoma. It is related to increased rate of cell death and differentiation of keratinocytes compare to normal skin. This kind of cell death is known as apoptosis. In this study, we plan to investigate the apoptotic cell death and expression of Fas in both normal and cholesteatoma epithelia. Materials and Methods:Seven cholesteatomas and retroauricular skins were obtained from patients undergoing middle ear operations. Detection of the fragmented DNA in apoptosis was done by in situ TUNEL methods and agarose gel electrophoresis. For the morphologic confirmation of apoptosis, transmission electron microscopy (TEM) was done. Immunohistochemistry was also performed for detection of Fas expression on the tissue. Results:In TUNEL staining, many positive staining nuclei were observed in upper layers of cholesteatoma epithelium whereas a few positive cells were found on the granular layer of retroauricular skin. Typical “ladder pattern” was seen on the gel electrophoresis of the genomic DNA of cholesteatoma. On TEM study, we observed condensation of chromatin in the keratinocytes of the cholesteatoma epithelium. Immunohistochemical studies revealed that Fas protein was expressed in all layers of cholesteatoma epithelium, while retroauricular skin showed weak reactions only in the granular layer. Conclusion:We confirmed that increased apoptosis and up-regulated expression of Fas in cholesteatoma epithelium. Since Fas is known as apoptosis triggering protein, the authors suggest that accumulation of keratin debris is due to increased apoptotic cell death and further investigation should be needed about the mechanism of cell death in cholesteatoma.-
dc.description.statementOfResponsibilityopen-
dc.languageKorean-
dc.publisher대한이비인후과학회-
dc.relation.isPartOfKorean Journal of Otolaryngology (대한이비인후과학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.title진주종상피에서 Apoptosis를 통한 세포의 사멸기전-
dc.title.alternativeMechanism of Apoptotic Cell Death in Cholesteatoma Epithelium-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학교실)-
dc.contributor.googleauthor박홍준-
dc.contributor.googleauthor박기현-
dc.contributor.googleauthor김보형-
dc.contributor.googleauthor정연훈-
dc.contributor.googleauthor정명현-
dc.contributor.localIdA03598-
dc.relation.journalcodeJ02088-
dc.contributor.alternativeNameChung, Myung Hyun-
dc.contributor.affiliatedAuthor정명현-
dc.citation.volume41-
dc.citation.number4-
dc.citation.startPage425-
dc.citation.endPage429-
dc.identifier.bibliographicCitationKorean Journal of Otolaryngology (대한이비인후과학회지), Vol.41(4) : 425-429, 1998-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.