Cited 11 times in
TNF-α Pretreatment Improves the Survival and Function of Transplanted Human Neural Progenitor Cells Following Hypoxic-Ischemic Brain Injury
DC Field | Value | Language |
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dc.contributor.author | 박국인 | - |
dc.contributor.author | 신정은 | - |
dc.date.accessioned | 2020-06-17T00:53:59Z | - |
dc.date.available | 2020-06-17T00:53:59Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 2073-4409 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176172 | - |
dc.description.abstract | Neural progenitor cells (NPCs) therapy offers great promise in hypoxic-ischemic (HI) brain injury. However, the poor survival of implanted NPCs in the HI host environment limits their therapeutic effects. Tumor necrosis factor-alpha (TNF-α) is a pleiotropic cytokine that is induced in response to a variety of pathological processes including inflammation and immunity. On the other hand, TNF-α has protective effects on cell apoptosis and death and affects the differentiation, proliferation, and survival of neural stem/progenitor cells in the brain. The present study investigated whether TNF-α pretreatment on human NPCs (hNPCs) enhances the effectiveness of cell transplantation therapy under ischemic brain. Fetal brain tissue-derived hNPCs were pretreated with TNF-α before being used in vitro experiments or transplantation. TNF-α significantly increased expression of cIAP2, and the use of short hairpin RNA-mediated knockdown of cIAP2 demonstrated that cIAP2 protected hNPCs against HI-induced cytotoxicity. In addition, pretreatment of hNPCs with TNF-α mediated neuroprotection by altering microglia polarization via increased expression of CX3CL1 and by enhancing expression of neurotrophic factors. Furthermore, transplantation of TNF-α-treated hNPCs reduced infarct volume and improved neurological functions in comparison with non-pretreated hNPCs or vehicle. These findings show that TNF-α pretreatment, which protects hNPCs from HI-injured brain-induced apoptosis and increases neuroprotection, is a simple and safe approach to improve the survival of transplanted hNPCs and the therapeutic efficacy of hNPCs in HI brain injury. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | CELLS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | TNF-α Pretreatment Improves the Survival and Function of Transplanted Human Neural Progenitor Cells Following Hypoxic-Ischemic Brain Injury | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pediatrics (소아청소년과학교실) | - |
dc.contributor.googleauthor | Miri Kim | - |
dc.contributor.googleauthor | Kwangsoo Jung | - |
dc.contributor.googleauthor | Younhee Ko | - |
dc.contributor.googleauthor | Il-Sun Kim | - |
dc.contributor.googleauthor | Kyujin Hwang | - |
dc.contributor.googleauthor | Jae-Hyung Jang | - |
dc.contributor.googleauthor | Jeong Eun Shin | - |
dc.contributor.googleauthor | Kook In Park | - |
dc.identifier.doi | 10.3390/cells9051195 | - |
dc.contributor.localId | A01438 | - |
dc.contributor.localId | A02152 | - |
dc.relation.journalcode | J03774 | - |
dc.identifier.pmid | 32403417 | - |
dc.subject.keyword | CX3CL1 | - |
dc.subject.keyword | cell survival | - |
dc.subject.keyword | cellular inhibitor of apoptosis 2 | - |
dc.subject.keyword | human neural progenitor cells | - |
dc.subject.keyword | hypoxic-ischemic brain injury | - |
dc.subject.keyword | tumor necrosis factor-alpha | - |
dc.contributor.alternativeName | Park, Kook In | - |
dc.contributor.affiliatedAuthor | 박국인 | - |
dc.contributor.affiliatedAuthor | 신정은 | - |
dc.citation.volume | 9 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | E1195 | - |
dc.identifier.bibliographicCitation | CELLS, Vol.9(5) : E1195, 2020-05 | - |
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