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PAX8 Expression in Anaplastic Thyroid Carcinoma Is Less Than Those Reported in Early Studies: A Multi-Institutional Study of 182 Cases Using the Monoclonal Antibody MRQ-50

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dc.contributor.author홍순원-
dc.date.accessioned2020-06-17T00:33:46Z-
dc.date.available2020-06-17T00:33:46Z-
dc.date.issued2020-03-
dc.identifier.issn0945-6317-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/176026-
dc.description.abstractAnaplastic thyroid carcinoma (ATC) is an aggressive malignant tumor composed of undifferentiated thyroid follicular cells. Pathological diagnosis of ATC can be challenging as the tumor may show morphological overlap with other neoplasms with anaplastic morphology. Immunohistochemical demonstration of thyroid origin facilitates the diagnosis of ATC. Previous studies using the polyclonal anti-PAX8 antibody 10336-1-AP suggested that PAX8 was the most sensitive marker, expressed in up to 80% of ATC. According to a 2018 NordiQC report, the monoclonal anti-PAX8 antibody MRQ-50 has become the most commonly used anti-PAX8 antibody worldwide. However, validation of this antibody in ATC is lacking. In this study, we recruited 182 ATC cases from seven institutions. Pathology slides were subjected to histology review. PAX8 immunohistochemistry using the MRQ-50 antibody was performed in whole tissue slides (n = 147) or tissue microarray sections (n = 35). We found PAX8 expression in 54.4% of the cases, which was significantly lower than those reported in prior studies with the polyclonal antibody. PAX8 expression was positively correlated with the presence of an epithelial pattern (63.6% vs 37.5%, p = 0.0008) and a coexisting differentiated thyroid carcinoma component (71.6% vs 44.3%, p = 0.0004), but was not associated with age, gender, specimen type, or presence of giant cell and sarcomatoid patterns. In conclusion, we demonstrated PAX8 expression using the monoclonal antibody MRQ-50 in only half of the cases in a large ATC series. Pathologists should be aware that PAX8 expression in ATC is less than those reported in early studies to avoid misdiagnosis.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherSpringer International-
dc.relation.isPartOfVIRCHOWS ARCHIV-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAdult-
dc.subject.MESHAged-
dc.subject.MESHAged, 80 and over-
dc.subject.MESHAntibodies, Monoclonal*-
dc.subject.MESHBiomarkers, Tumor / analysis*-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHImmunohistochemistry / methods-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHPAX8 Transcription Factor / analysis-
dc.subject.MESHPAX8 Transcription Factor / biosynthesis*-
dc.subject.MESHThyroid Carcinoma, Anaplastic / diagnosis*-
dc.subject.MESHThyroid Neoplasms / diagnosis*-
dc.titlePAX8 Expression in Anaplastic Thyroid Carcinoma Is Less Than Those Reported in Early Studies: A Multi-Institutional Study of 182 Cases Using the Monoclonal Antibody MRQ-50-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pathology (병리학교실)-
dc.contributor.googleauthorWei-An Lai-
dc.contributor.googleauthorJen-Fan Hang-
dc.contributor.googleauthorChih-Yi Liu-
dc.contributor.googleauthorYanhua Bai-
dc.contributor.googleauthorZhiyan Liu-
dc.contributor.googleauthorHaiyan Gu-
dc.contributor.googleauthorSoonWon Hong-
dc.contributor.googleauthorJu Yeon Pyo-
dc.contributor.googleauthorChan Kwon Jung-
dc.contributor.googleauthorKennichi Kakudo-
dc.contributor.googleauthorAndrey Bychkov-
dc.identifier.doi10.1007/s00428-019-02708-4-
dc.contributor.localIdA04411-
dc.relation.journalcodeJ02784-
dc.identifier.eissn1432-2307-
dc.identifier.pmid31732814-
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs00428-019-02708-4-
dc.subject.keywordAnaplastic thyroid carcinoma (ATC)-
dc.subject.keywordImmunohistochemistry-
dc.subject.keywordMRQ-50-
dc.subject.keywordPaired box gene 8 (PAX8)-
dc.subject.keywordUndifferentiated thyroid carcinoma-
dc.contributor.alternativeNameHong, Soon Won-
dc.contributor.affiliatedAuthor홍순원-
dc.citation.volume476-
dc.citation.number3-
dc.citation.startPage431-
dc.citation.endPage437-
dc.identifier.bibliographicCitationVIRCHOWS ARCHIV, Vol.476(3) : 431-437, 2020-03-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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