Cited 11 times in
Simultaneous Analysis of Multiple Cancer Biomarkers Using MALDI-TOF Mass Spectrometry Based on a Parylene-Matrix Chip
DC Field | Value | Language |
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dc.contributor.author | 이상국 | - |
dc.date.accessioned | 2020-06-17T00:26:05Z | - |
dc.date.available | 2020-06-17T00:26:05Z | - |
dc.date.issued | 2020-04 | - |
dc.identifier.issn | 1044-0305 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/175965 | - |
dc.description.abstract | Recently, the parylene-matrix chip was developed for quantitative analysis of small molecules less than 1 kDa. In this study, MALDI-TOF MS based on the parylene-matrix chip was performed to clinically diagnose intrahepatic cholangiocarcinoma (IHCC) and colorectal cancer (CRC). The parylene-matrix chip was applied for the detection of small cancer biomarkers, including N-methyl-2-pyridone-5-carboxamide (2PY), glutamine, lysophosphatidylcholine (LPC) 16:0, and LPC 18:0. The feasibility of MALDI-TOF MS based on the parylene-matrix chip was confirmed via analysis of spot-to-spot and shot-to-shot reproducibility. Serum metabolite markers of IHCC, N-methyl-2-pyridone-5-carboxamide (2PY), and glutamine were quantified using MALDI-TOF MS based on the parylene-matrix chip. For clinical diagnosis of CRC, two water-insoluble (barely soluble) biomarkers, lysophosphatidylcholine (LPC) 16:0 and LPC 18:0, were quantified. Finally, glutamine and LPC 16:0 were simultaneously detected at a range of concentrations in sera from colon cancer patients using the parylene-matrix chip. Thus, this method yielded high-throughput detection of cancer biomarkers for the mixture samples of water-soluble analytes (2PY and glutamine) and water-insoluble analytes (LPC 16:0 and LPC 18:0). | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | ACS Publications | - |
dc.relation.isPartOf | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Simultaneous Analysis of Multiple Cancer Biomarkers Using MALDI-TOF Mass Spectrometry Based on a Parylene-Matrix Chip | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Jong-Min Park | - |
dc.contributor.googleauthor | Moon-Ju Kim | - |
dc.contributor.googleauthor | Joo-Yoon Noh | - |
dc.contributor.googleauthor | Tae Gyeong Yun | - |
dc.contributor.googleauthor | Min-Jung Kang | - |
dc.contributor.googleauthor | Sang-Guk Lee | - |
dc.contributor.googleauthor | Byong Chul Yoo | - |
dc.contributor.googleauthor | Jae-Chul Pyun | - |
dc.identifier.doi | 10.1021/jasms.9b00102 | - |
dc.contributor.localId | A02810 | - |
dc.relation.journalcode | J03808 | - |
dc.identifier.eissn | 1879-1123 | - |
dc.identifier.pmid | 32154716 | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/jasms.9b00102 | - |
dc.subject.keyword | N-methyl-2-pyridone-5-carboxamide | - |
dc.subject.keyword | cancer biomarker | - |
dc.subject.keyword | glutamine | - |
dc.subject.keyword | lysophosphatidylcholine | - |
dc.subject.keyword | matrix-assisted laser desorption/ionization time-of-flight mass spectrometry | - |
dc.subject.keyword | parylene-matrix chip | - |
dc.contributor.alternativeName | Lee, Sang Guk | - |
dc.contributor.affiliatedAuthor | 이상국 | - |
dc.citation.volume | 31 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 917 | - |
dc.citation.endPage | 926 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol.31(4) : 917-926, 2020-04 | - |
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