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Simultaneous Analysis of Multiple Cancer Biomarkers Using MALDI-TOF Mass Spectrometry Based on a Parylene-Matrix Chip

Authors
 Jong-Min Park  ;  Moon-Ju Kim  ;  Joo-Yoon Noh  ;  Tae Gyeong Yun  ;  Min-Jung Kang  ;  Sang-Guk Lee  ;  Byong Chul Yoo  ;  Jae-Chul Pyun 
Citation
 JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol.31(4) : 917-926, 2020-04 
Journal Title
 JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 
ISSN
 1044-0305 
Issue Date
2020-04
Keywords
N-methyl-2-pyridone-5-carboxamide ; cancer biomarker ; glutamine ; lysophosphatidylcholine ; matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ; parylene-matrix chip
Abstract
Recently, the parylene-matrix chip was developed for quantitative analysis of small molecules less than 1 kDa. In this study, MALDI-TOF MS based on the parylene-matrix chip was performed to clinically diagnose intrahepatic cholangiocarcinoma (IHCC) and colorectal cancer (CRC). The parylene-matrix chip was applied for the detection of small cancer biomarkers, including N-methyl-2-pyridone-5-carboxamide (2PY), glutamine, lysophosphatidylcholine (LPC) 16:0, and LPC 18:0. The feasibility of MALDI-TOF MS based on the parylene-matrix chip was confirmed via analysis of spot-to-spot and shot-to-shot reproducibility. Serum metabolite markers of IHCC, N-methyl-2-pyridone-5-carboxamide (2PY), and glutamine were quantified using MALDI-TOF MS based on the parylene-matrix chip. For clinical diagnosis of CRC, two water-insoluble (barely soluble) biomarkers, lysophosphatidylcholine (LPC) 16:0 and LPC 18:0, were quantified. Finally, glutamine and LPC 16:0 were simultaneously detected at a range of concentrations in sera from colon cancer patients using the parylene-matrix chip. Thus, this method yielded high-throughput detection of cancer biomarkers for the mixture samples of water-soluble analytes (2PY and glutamine) and water-insoluble analytes (LPC 16:0 and LPC 18:0).
Full Text
https://pubs.acs.org/doi/10.1021/jasms.9b00102
DOI
10.1021/jasms.9b00102
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Sang-Guk(이상국) ORCID logo https://orcid.org/0000-0003-3862-3660
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/175965
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