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Investigation of PRDM7 and PRDM12 Expression Pattern During Mouse Embryonic Development by Using a Modified Passive Clearing Technique

Authors
 Jiwon Woo  ;  Haewon Kang  ;  Eunice Yoojin Lee  ;  Sangkyu Park  ;  Yong Eun Cho 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.524(2) : 346-353, 2020-04 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2020-04
Keywords
PRDM12 ; PRDM7 ; Tissue clearing ; Transparent-embryo ; mPACT-A
Abstract
Recent developments in tissue clearing methods such as CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue hYdrogel) have allowed for the three-dimensional analysis of biological structures in whole, intact tissue, providing greater understanding of spatial relationships and biological circuits. Nonetheless, studies have reported issues with maintaining structural integrity and preventing tissue disintegration, preventing the wide application of these techniques to fragile tissues such as developing embryos. Here, we present optimized passive clearing techniques, mPACT-A, that improve tissue rigidity without the expense of optical transparency. We also present a further modified mPACT-A protocol that is specifically optimized for handling mouse embryos, which are small and fragile, such that they easily dismantle when processed via established tissue clearing methods. We demonstrate proof-of-concept by investigating the expression of two relatively understudied PRDM proteins, PRDM7 and PRDM12, in intact cleared mouse embryos at various stages of development. We observed strong PRDM7 and PRDM12 expression in the developing mouse nervous system, suggestive of potential roles in neural development that will be tested in future functional studies.
Full Text
https://www.sciencedirect.com/science/article/pii/S0006291X20301017
DOI
10.1016/j.bbrc.2019.12.133
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Cho, Yong Eun(조용은) ORCID logo https://orcid.org/0000-0001-9815-2720
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/175945
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