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Transcriptional Expression in Human Periodontal Ligament Cells Subjected to Orthodontic Force: An RNA-Sequencing Study

Authors
 Kyunam Kim  ;  Hee Eun Kang  ;  Jong In Yook  ;  Hyung-Seog Yu  ;  Euiseong Kim  ;  Jung-Yul Cha  ;  Yoon Jeong Choi 
Citation
 JOURNAL OF CLINICAL MEDICINE, Vol.9(2) : E358, 2020 
Journal Title
 JOURNAL OF CLINICAL MEDICINE 
Issue Date
2020
Keywords
RNA-sequencing ; mechanical stimulus ; orthodontic force ; periodontal ligament ; prospective study
Abstract
This study was performed to investigate the changes in gene expression in periodontal ligament (PDL) cells following mechanical stimulus through RNA sequencing. In this study, premolars extracted for orthodontic treatment were used. To stimulate the PDL cells, an orthodontic force of 100× g was applied to the premolar (experimental group; n = 11), whereas the tooth on the other side was left untreated (control group; n = 11). After the PDL cells were isolated from the extracted teeth, gene set enrichment analysis (GSEA), differentially expressed gene (DEG) analysis, and real-time PCR were performed to compare the two groups. GSEA demonstrated that gene sets related to the cell cycle pathway were upregulated in PDL. Thirteen upregulated and twenty downregulated genes were found through DEG analysis. Real-time PCR results confirmed that five upregulated genes (CC2D1B, CPNE3, OPHN1, TANGO2, and UAP-1) and six downregulated genes (MYOM2, PPM1F, PCDP1, ATP2A1, GPR171, and RP1-34H18.1-1) were consistent with RNA sequencing results. We suggest that, from among these eleven genes, two upregulated genes, CPNE3 and OPHN1, and one downregulated gene, PPM1F, play an important role in PDL regeneration in humans when orthodontic force is applied.
Files in This Item:
T202000333.pdf Download
DOI
10.3390/jcm9020358
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Yu, Hyung Seog(유형석) ORCID logo https://orcid.org/0000-0002-1275-5078
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
Cha, Jung Yul(차정열)
Choi, Yoon Jeong(최윤정) ORCID logo https://orcid.org/0000-0003-0781-8836
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/175299
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