Cited 8 times in
Performance Evaluation of a New Automated Chemiluminescent Immunoanalyzer-Based Interferon-Gamma Releasing Assay AdvanSure I3 in Comparison With the QuantiFERON-TB Gold In-Tube Assay
DC Field | Value | Language |
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dc.contributor.author | 김현숙 | - |
dc.contributor.author | 박윤희 | - |
dc.date.accessioned | 2020-02-11T06:55:47Z | - |
dc.date.available | 2020-02-11T06:55:47Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/174904 | - |
dc.description.abstract | BACKGROUND: The interferon-gamma (IFN-γ) releasing assay (IGRA) is widely used for latent tuberculosis infection (LTBI) diagnosis. We evaluated the analytical performance of a new automated chemiluminescent immunoanalyzer-based IGRA (CLIA-IGRA), AdvanSure I3 (LG Life Sciences, Seoul, Korea) and compared it with that of the QuantiFERON-TB Gold In-Tube (QFT-GIT) assay. METHODS: Repeatability and reproducibility were evaluated at four levels. Detection capability, including limit of blank (LoB), limit of detection (LoD), and limit of quantification (LoQ), was evaluated using IFN-γ standard material (National Institute for Biological Standards and Control code: 87/586). Agreement between the results of two assays was evaluated using 341 blood samples from healthcare workers and patients at a tertiary care hospital. To determine the cut-off value of CLIA-IGRA for diagnosing LTBI, the ROC curve was analyzed. RESULTS: Repeatability and reproducibility were 4.86-7.00% and 6.36-7.88% CV, respectively. LoB, LoD, and LoQ were 0.022, 0.077, and 0.249 IU/mL, respectively. IFN-γ values between CLIA-IGRA and QFT-GIT showed a strong correlation within the analytical measurable range of both assays, especially when the value was low. Qualitative comparison of the two assays yielded a 99.1% overall agreement (kappa coefficient=0.98). A cut-off value of 0.35 IU/mL was appropriate for diagnosing LTBI. CONCLUSIONS: CLIA-IGRA is a reliable assay for LTBI diagnosis, with performance similar to that of QFT-GIT. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Korean Society for Laboratory Medicine | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Performance Evaluation of a New Automated Chemiluminescent Immunoanalyzer-Based Interferon-Gamma Releasing Assay AdvanSure I3 in Comparison With the QuantiFERON-TB Gold In-Tube Assay | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Jin Ju Kim | - |
dc.contributor.googleauthor | Younhee Park | - |
dc.contributor.googleauthor | Dasom Choi | - |
dc.contributor.googleauthor | Hyon-Suk Kim | - |
dc.identifier.doi | 10.3343/alm.2020.40.1.33 | - |
dc.contributor.localId | A01117 | - |
dc.contributor.localId | A01606 | - |
dc.relation.journalcode | J00164 | - |
dc.identifier.eissn | 2234-3814 | - |
dc.identifier.pmid | 31432637 | - |
dc.subject.keyword | Chemiluminescent immunoanalyzer | - |
dc.subject.keyword | Interferon-γ releasing assay | - |
dc.subject.keyword | Latent tuberculosis infection | - |
dc.subject.keyword | Performance | - |
dc.subject.keyword | QuantiFERON-TB Gold In-Tube | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.affiliatedAuthor | 김현숙 | - |
dc.contributor.affiliatedAuthor | 박윤희 | - |
dc.citation.volume | 40 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 33 | - |
dc.citation.endPage | 39 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, Vol.40(1) : 33-39, 2020 | - |
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