Cited 17 times in
Cancer-associated fibroblast stimulates cancer cell invasion in an interleukin-1 receptor (IL-1R)-dependent manner
DC Field | Value | Language |
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dc.contributor.author | 장향란 | - |
dc.date.accessioned | 2020-02-11T06:08:04Z | - |
dc.date.available | 2020-02-11T06:08:04Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 1792-1074 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/174549 | - |
dc.description.abstract | Tumor microenvironment serves an important role in tumor growth and metastasis. Cancer cells can promote growth and malignancy by altering the surrounding stroma. Cancer-associated fibroblast (CAF) are an abundant cell type present within the tumor microenvironment and provide tumorigenic features by secreting cytokines. In the current study, the CAF-mediated invasion of oral squamous cell carcinoma (OSCC) was investigated and the associated mechanisms were elucidated. Cancer invasion was estimated using a Matrigel-coated Transwell chamber and FITC-gelatin matrix. To verify the effect of the tumor microenvironment, conditioned media (CM) from normal fibroblast (NF) and CAFs were prepared. An ELISA was performed to estimate the level of IL-1β. A proteome profiler human protease array was performed to verify the proteases affected by stimulation with CM, from CAF. Recombinant IL-1β protein increased the invasion of OSCC cells. IL-1β expression was higher in CAF than NF. CM from CAF (CM-CAF) increased cancer invasion and FITC-gelatin matrix degradation. The invasive capacity provided by CAF was abrogated by an IL-1 receptor (IL-1R) antagonist. Additionally, CM-CAF increased the secretion of ADAM 9 and Kallikrein 11 from OSCC cells. The invasion activity by CM-CAF was partially abrogated by the neutralization of ADAM 9 or Kallikrein 11. In conclusion, by providing stromal factor, CAFs were a critical inducer of OSCC invasion, and CAF secretes the required amount of IL-1β to increase cancer invasion activity. The invasive capacity of CAF was identified to be IL-1R-dependent. ADAM 9 and Kallikrein 11 were influencing factors involved in the increase of CAF-mediated cancer invasion. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Spandidos Publications | - |
dc.relation.isPartOf | ONCOLOGY LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Cancer-associated fibroblast stimulates cancer cell invasion in an interleukin-1 receptor (IL-1R)-dependent manner | - |
dc.type | Article | - |
dc.contributor.college | Research Institutes (연구소) | - |
dc.contributor.department | Oral Cancer Research Institute (구강종양연구소) | - |
dc.contributor.googleauthor | Xianglan Zhang | - |
dc.contributor.googleauthor | Young Sun Hwang | - |
dc.identifier.doi | 10.3892/ol.2019.10784 | - |
dc.contributor.localId | A03489 | - |
dc.relation.journalcode | J02417 | - |
dc.identifier.eissn | 1792-1082 | - |
dc.identifier.pmid | 31611973 | - |
dc.subject.keyword | cancer-associated fibroblast | - |
dc.subject.keyword | interleukin-1β | - |
dc.subject.keyword | invasion | - |
dc.subject.keyword | protease | - |
dc.subject.keyword | tumor microenvironment | - |
dc.contributor.alternativeName | Zhang, Xiang Lan | - |
dc.contributor.affiliatedAuthor | 장향란 | - |
dc.citation.volume | 18 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 4645 | - |
dc.citation.endPage | 4650 | - |
dc.identifier.bibliographicCitation | ONCOLOGY LETTERS, Vol.18(5) : 4645-4650, 2019 | - |
dc.identifier.rimsid | 63565 | - |
dc.type.rims | ART | - |
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