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An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence
DC Field | Value | Language |
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dc.contributor.author | 조용은 | - |
dc.date.accessioned | 2020-02-11T06:05:12Z | - |
dc.date.available | 2020-02-11T06:05:12Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/174527 | - |
dc.description.abstract | The SARS-CoV nucleocapsid (N) protein serves multiple functions in viral replication, transcription, and assembly of the viral genome complex. Coronaviruses specifically package genomic RNA into assembled virions, and in SARS-CoV, it is reported that this process is driven by an interaction between the N-protein and a packaging signal encoded within the viral RNA. While recent studies have uncovered the sequence of this packaging signal, little is known about the specific interaction between the N-protein and the packaging signal sequence, and the mechanisms by which this interaction drives viral genome packaging. In this study, we developed a novel in vivo cell-based assay for examining this interaction between the N-protein and packaging signal RNA for SARS-CoV, as well as other viruses within the coronaviridae family. Our results demonstrate that the N-protein specifically recognizes the SARS-CoV packaging signal with greater affinity compared to signals from other coronaviruses or non-coronavirus species. We also use deletion mapping to identify a 151-nt region within the packaging signal sequence that is critical for N-protein-RNA binding, and conversely, we show that both the N-terminal and C-terminal domains of the N protein are necessary for recognizing the packaging RNA. These results describe, for the first time, in vivo evidence for an interaction between the SARS-CoV N-protein and its packaging signal RNA, and demonstrate the feasibility of using this cell-based assay to further probe viral RNA-protein interactions in future studies. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학교실) | - |
dc.contributor.googleauthor | Jiwon Woo | - |
dc.contributor.googleauthor | Eunice Yoojin Lee | - |
dc.contributor.googleauthor | Mirae Lee | - |
dc.contributor.googleauthor | Taeyeon Kim | - |
dc.contributor.googleauthor | Yong-Eun Cho | - |
dc.identifier.doi | 10.1016/j.bbrc.2019.09.115 | - |
dc.contributor.localId | A03865 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 31594639 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0006291X19318534 | - |
dc.subject.keyword | Cell-based assay | - |
dc.subject.keyword | Coronavirus | - |
dc.subject.keyword | Nucleocapsid | - |
dc.subject.keyword | Packaging signal sequence | - |
dc.subject.keyword | Protein-RNA interaction | - |
dc.subject.keyword | SARS | - |
dc.contributor.alternativeName | Cho, Yong Eun | - |
dc.contributor.affiliatedAuthor | 조용은 | - |
dc.citation.volume | 520 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 499 | - |
dc.citation.endPage | 506 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.520(3) : 499-506, 2019 | - |
dc.identifier.rimsid | 63406 | - |
dc.type.rims | ART | - |
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