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식사 및 검체 보관이 면역 측정법에 의한 저비중지단백 콜레스테롤 직접 측정법에 미치는 영향
DC Field | Value | Language |
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dc.contributor.author | 김정호 | - |
dc.date.accessioned | 2020-01-23T05:17:06Z | - |
dc.date.available | 2020-01-23T05:17:06Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 1015-6445 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/174153 | - |
dc.description.abstract | Background : Elevated level of low density lipoprotein-cholesterol (LDL-C) is one of the major risk factors for the development of coronary heart disease. Direct LDL-C determination method by immunoseparation (DLDL-C) recently developed is claimed not to be influenced by food ingestion. We re-evaluated the effects of diet and storage conditions for this method. Methods : Samples were collected from thirty-two medical college students before and after meal to study the effects of diet on this method. We compared the difference of LDL-C of filtered samples between refrigerated and frozen state. We also compared direct and indirect calculated measurements of LDL-C with ultracentrifugal beta-quantification (BQLDL-C) method. Results : Morning 2-hour-postprandial specimen can be acceptable with no minimal significant bias, but afternoon 2-hour or 4-hour-postprandial specimen cannot be recommended due to significant negative bias (8.6-9.6%). Storage of filtered samples showed no significant difference between frozen and refrigerated state. Calculated LDL-C when triglyceride level is more than 400 mg/dL was not reliable due to large proportional and constant bias. In contrast, DLDL-C showed good accuracy comparing with BQLDL-C (y=0.909x+3.3, r=0.869, n=9, x=BQLDL-C, y=DLDL-C). Conclusions : In conclusion, morning two-hour postprandial specimens can be acceptable for DLDL-C, but afternoon postprandial specimens may not be recommended due to significant negative bias. DLDL-C seems to be reliable and useful especially for hypertriglyceridemic patients or follow-up cases of hypercholesterolemia with normal triglyceride or HDL-C levels. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 대한임상병리학회 | - |
dc.relation.isPartOf | Korean Journal of Clinical Pathology (대한임상병리학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | 식사 및 검체 보관이 면역 측정법에 의한 저비중지단백 콜레스테롤 직접 측정법에 미치는 영향 | - |
dc.title.alternative | The Influence of Food Ingestion and Sample Storage on Direct LDL-Cholesterol Measurement by Immunoseparation Method | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | 임환섭, 정재림, 박광일, 김정호, 권오헌 | - |
dc.contributor.localId | A00903 | - |
dc.relation.journalcode | J01988 | - |
dc.subject.keyword | Low density lipoprotein cholesterol | - |
dc.subject.keyword | lmmunoseparation | - |
dc.subject.keyword | Direct LDL-C measurement | - |
dc.subject.keyword | Beta-Quantification | - |
dc.subject.keyword | Ultracentrifugation | - |
dc.subject.keyword | Fasting | - |
dc.contributor.alternativeName | Kim, Jeong Ho | - |
dc.contributor.affiliatedAuthor | 김정호 | - |
dc.citation.volume | 19 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 40 | - |
dc.citation.endPage | 45 | - |
dc.identifier.bibliographicCitation | Korean Journal of Clinical Pathology (대한임상병리학회지), Vol.19(1) : 40-45, 1999 | - |
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