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Regulation of type I collagen and interstitial collagenase mRNA expression in human dermal fibroblasts by colchicine and D-penicillamine

Authors
 Kee Yang Chung  ;  Dong Seung Kang 
Citation
 Yonsei Medical Journal, Vol.40(5) : 490-495, 1999 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
1999
MeSH
Cells, Cultured ; Colchicine/pharmacology* ; Collagen/genetics* ; Fibroblasts/metabolism ; Gene Expression Regulation/drug effects* ; Humans ; Matrix Metalloproteinase 1/genetics* ; Penicillamine/pharmacology* ; RNA, Messenger/analysis* ; Skin/cytology ; Skin/metabolism ; Transforming Growth Factor beta/pharmacology
Abstract
Sclerosis is a disease process in which idiopathic hardening occurs in the skin and/or internal organs as a result of the accumulation of type I collagen, induced mainly by transforming growth factor-beta. Colchicine and D-penicillamine are widely used for its treatment. Their effects are known to be due to post-translational down-regulation of type I collagen synthesis, with colchicine also up-regulating interstitial collagenase. To determine whether or not they have any pre-translational effect on type I collagen and MMP-1, and also to observe their effects on the action of TGF-beta, cultured neonatal foreskin fibroblasts were treated with colchicine and D-penicillamine, singly and together. The amount of type I collagen and MMP-1 mRNA were quantitated by Northern blot hybridization. Colchicine suppresses the basal level of type I collagen mRNA but minimally stimulates the mRNA expression of MMP-1, whereas D-penicillamine does not have any significant effects on either. Colchicine was also able to significantly suppress the TGF-beta-induced up-regulation of type I collagen mRNA expression.
Files in This Item:
T199901212.pdf Download
DOI
10.3349/ymj.1999.40.5.490
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Chung, Kee Yang(정기양) ORCID logo https://orcid.org/0000-0003-3257-0297
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173973
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