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Pyrithione, a zinc ionophore, inhibits NF-kappaB activation

Authors
 Chul Hoon Kim  ;  Joo Hee Kim  ;  Seok Jun Moon  ;  Kwang Chul Chung  ;  Chung Y. Hsu  ;  Jeong Taeg Seo  ;  Young Soo Ahn 
Citation
 Biochemical and Biophysical Research Communications, Vol.259(3) : 505-509, 1999 
Journal Title
 Biochemical and Biophysical Research Communications 
ISSN
 0006-291X 
Issue Date
1999
MeSH
Animals ; Calcium/metabolism ; Cattle ; Cells, Cultured ; Dose-Response Relationship, Drug ; Enzyme Activation/drug effects ; Ionophores/pharmacology* ; NF-kappa B/metabolism* ; Pyridines/pharmacology* ; Pyrrolidines/pharmacology* ; Tetradecanoylphorbol Acetate/pharmacology ; Thiocarbamates/pharmacology* ; Thiones ; Time Factors ; Tumor Necrosis Factor-alpha/pharmacology ; Zinc/metabolism*
Abstract
Pyrrolidine dithiocarbamate (PDTC) suppresses NF-kappaB activity and exhibits cytotoxic effects in bovine cerebral endothelial cells (BCECs), and we have previously reported that these PDTC effects were accompanied by an increase in intracellular zinc levels. To further explore the role of zinc in the modulation of NF-kappaB activation, we studied the effect of pyrithione, a zinc ionophore, on NF-kappaB activation in BCECs. Pyrithione inhibited NF-kappaB activity in a time- and dose-dependent manner. Ca-EDTA, but not Zn-EDTA, prevented pyrithione inhibition of NF-kappaB activity. Pyrithione increased the intracellular zinc level within 15 min. This effect was also abolished by Ca-EDTA, but not by Zn-EDTA. The potency of pyrithione on NF-kappaB inhibition and zinc influx was approximately one order of magnitude more potent than PDTC. These findings establish the regulatory role of intracellular zinc levels on NF-kappaB activity in BCECs.
Full Text
https://www.sciencedirect.com/science/article/pii/S0006291X99908148
DOI
10.1006/bbrc.1999.0814
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
Ahn, Young Soo(안영수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173947
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