0 11

Cited 0 times in

Effects of TNF-alpha and IL-1 beta on mucin, lysozyme, IL-6 and IL-8 in passage-2 normal human nasal epithelial cells

Authors
 Joo-Heon Yoon  ;  Kyung-Su Kim  ;  Hyun Ung Kim  ;  John Alderman Linton  ;  Jeung-Gweon Lee 
Citation
 Acta Oto-Laryngologica, Vol.119(8) : 905-910, 1999 
Journal Title
 Acta Oto-Laryngologica 
ISSN
 0001-6489 
Issue Date
1999
MeSH
Cells, Cultured ; Epithelium/metabolism ; Humans ; Immunologic Techniques ; Interleukin-1/pharmacology* ; Interleukin-6/genetics ; Interleukin-6/metabolism* ; Interleukin-8/genetics ; Interleukin-8/metabolism* ; Mucins/genetics ; Mucins/metabolism* ; Muramidase/genetics ; Muramidase/metabolism* ; Nasal Mucosa/metabolism* ; RNA, Messenger/analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Necrosis Factor-alpha/pharmacology*
Abstract
Little is known about the regulatory effects of cytokines on various nasal secretions in normal human nasal epithelial cells. The aim of this study was to examine whether TNF-alpha, IL-1beta or their combination can increase the secretion of mucin as an indicator of mucous secretion, the secretion of lysozyme as an indicator of serous secretion and the secretion of IL-6 and IL-8 as important cytokines. In addition, we wanted to examine their message levels in normal human nasal epithelium. On day 12 of culture, passage-2 normal human nasal epithelial cells were treated with 10 ng/ml TNF-alpha, 10 ng/ml IL-1beta and combinations of both. Twenty-four hours later, the apical secretions were collected. A mixture of TNF-alpha and IL-1beta synergistically increased secretion of mucin, IL-6 and IL-8, but did not increase secretion of lysozyme. A combination of TNF-alpha and IL-1beta showed a questionable increase of MUC2 mRNA levels. TNF-alpha, IL-1beta and a combination of both all significantly increased MUC8 mRNA levels. Neither TNF-alpha, IL-1beta nor a combination of both increased MUC5AC, MUC5B and lysozyme mRNA levels. IL-1beta alone or a combination of TNF-alpha and IL-1beta comparably increased IL-6 and IL-8 mRNA levels slightly. In conclusion, a mixture of inflammatory mediators can synergistically increase secretion of mucin, IL-6 and IL-8 in human nasal epithelium. Accordingly, nasal secretions may be under the control of an inflammatory mediator network.
Full Text
https://www.tandfonline.com/doi/abs/10.1080/00016489950180261
DOI
10.1080/00016489950180261
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173867
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse