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MALDI-TOF Mass Spectrometry Based on Parylene-Matrix Chip for the Analysis of Lysophosphatidylcholine in Sepsis Patient Sera

 Jong-Min Park  ;  Joo-Yoon Noh  ;  Moon-Ju Kim  ;  Tae Gyeong Yun  ;  Sang-Guk Lee  ;  Kyung Soo Chung  ;  Eun Hye Lee  ;  Mi Hwa Shin  ;  Nam Su Ku  ;  Sohee Yoon  ;  Min-Jung Kang  ;  Moo Suk Park  ;  Jae-Chul Pyun 
 ANALYTICAL CHEMISTRY, Vol.91(22) : 14719-14727, 2019 
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In this work, medical diagnosis of sepsis was conducted via quantitative analysis of lysophosphatidylcholine 16:0 (LPC 16:0) by using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry based on a parylene-matrix chip. In the first step, specific mass peaks for the diagnosis of sepsis were searched by comparing MALDI-TOF mass spectra of sepsis patient sera with healthy controls and pneumonia patient sera. Two mass peaks at m/z = 496.3 and 518.3 were chosen as those that are specifically different for sepsis sera to compare with healthy controls and pneumonia patient sera. These mass peaks were identified to be protonated and sodium adducts of LPC 16:0 by using tandem mass spectra (MS2 and MS3) of purely synthesized LPC 16:0 and extracted LPC 16:0 from a healthy control and a sepsis patient. In the next step, a standard curve for LPC 16:0 for the quantitative analysis of LPC 16:0 with MALDI-TOF MS based on the parylene-matrix chip was prepared, and the statistical correlation to the LC-MS analysis results was demonstrated by using the Bland-Altman test and Passing-Bablok regression. Finally, MALDI-TOF MS based on the parylene-matrix chip was used for the quantification of LPC 16:0 with sera from patients with severe sepsis and septic shock (n = 143), pneumonia patients (n = 12), and healthy sera (n = 31). The sensitivity and the selectivity of medical diagnosis of sepsis was estimated to be 97.9% and 95.5% by using MALDI-TOF MS based on the parylene-matrix chip, respectively.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Ku, Nam Su(구남수) ORCID logo https://orcid.org/0000-0002-9717-4327
Park, Moo Suk(박무석) ORCID logo https://orcid.org/0000-0003-0820-7615
Shin, Mi Hwa(신미화) ORCID logo https://orcid.org/0000-0003-2215-8629
Lee, Sang-Guk(이상국) ORCID logo https://orcid.org/0000-0003-3862-3660
Lee, Eun Hye(이은혜) ORCID logo https://orcid.org/0000-0003-2570-3442
Jung, Kyung Soo(정경수) ORCID logo https://orcid.org/0000-0003-1604-8730
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