Cited 9 times in
Detection of Helicobacter pylori with clarithromycin resistance-associated mutations using peptide nucleic acid probe-based melting point analysis
DC Field | Value | Language |
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dc.contributor.author | 김현기 | - |
dc.contributor.author | 남지해 | - |
dc.contributor.author | 김원규 | - |
dc.date.accessioned | 2019-12-18T01:10:16Z | - |
dc.date.available | 2019-12-18T01:10:16Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 1083-4389 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/173401 | - |
dc.description.abstract | BACKGROUND: Detection of Helicobacter pylori in gastric biopsy is important for appropriate treatment and prevention of gastric carcinoma and lymphoma. A novel peptide nucleic acid probe (PNA)-based real-time polymerase chain reaction (PCR) method was developed for detection of H pylori and A2142G/A2143G mutation of the 23S rRNA gene, which is associated with clarithromycin resistance. METHODS: To evaluate the performance of the PNA probe-based PCR method, a total of 409 gastric biopsy samples were analyzed by PNA probe-based PCR and compared with other H pylori detection methods, including hematoxylin and eosin (HE) and Warthin-Starry (WS) staining, immunohistochemistry (IHC). A2142G/A2143G mutation of the 23S rRNA gene was tested by dual priming oligonucleotide (DPO)-based PCR and Sanger sequencing to evaluate PNA probe-based PCR. RESULTS: Among 271 cases that were positive for H pylori on IHC which was considered as a standard method, 264 cases (97.4%) and 259 cases (95.6%) were positively detected by HE/WS and PNA probe-based qPCR, respectively. Of 100 H pylori-positive patients tested by IHC, H pylori was detected in 93 cases (93.0%) by PNA probe-based PCR, 86 cases (86.0%) by DPO-based PCR, and 93 cases (93.0%) by conventional PCR. The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of PNA probe-based qPCR were 93.0%, 94.9%, 93.9%, 94.9%, and 93.0%, respectively, which were all higher than those of DPO-based PCR. When Sanger sequencing was determined as a standard method to detect A2142G/A2143G mutations, the sensitivity of the PNA- and DPO-based methods was 100% and 94.4%, respectively, and the specificity was 100% for both methods. CONCLUSION: PNA probe-based qPCR is an appropriate method for detecting H pylori and the clarithromycin resistance-associated mutation type. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.relation.isPartOf | HELICOBACTER | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Detection of Helicobacter pylori with clarithromycin resistance-associated mutations using peptide nucleic acid probe-based melting point analysis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학교실) | - |
dc.contributor.googleauthor | Ji Hae Nahm | - |
dc.contributor.googleauthor | Won Kyu Kim | - |
dc.contributor.googleauthor | Yujin Kwon | - |
dc.contributor.googleauthor | Hyunki Kim | - |
dc.identifier.doi | 10.1111/hel.12634 | - |
dc.contributor.localId | A01108 | - |
dc.relation.journalcode | J00981 | - |
dc.identifier.eissn | 1523-5378 | - |
dc.identifier.pmid | 31304664 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/full/10.1111/hel.12634 | - |
dc.subject.keyword | Helicobacter pylori diagnosis | - |
dc.subject.keyword | clarithromycin resistance | - |
dc.subject.keyword | gastric biopsy | - |
dc.subject.keyword | immunohistochemistry | - |
dc.subject.keyword | peptide nucleic acid probe | - |
dc.subject.keyword | real-time polymerase chain reaction | - |
dc.contributor.alternativeName | Kim, Hyunki | - |
dc.contributor.affiliatedAuthor | 김현기 | - |
dc.citation.volume | 24 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | e12634 | - |
dc.identifier.bibliographicCitation | HELICOBACTER, Vol.24(5) : e12634, 2019 | - |
dc.identifier.rimsid | 64050 | - |
dc.type.rims | ART | - |
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