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Performance Evaluation of the Newly Developed BD Phoenix NMIC-500 Panel Using Clinical Isolates of Gram-Negative Bacilli

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dc.contributor.author김도균-
dc.contributor.author이혁민-
dc.contributor.author정석훈-
dc.contributor.author윤은정-
dc.contributor.author홍준성-
dc.date.accessioned2019-12-18T00:57:39Z-
dc.date.available2019-12-18T00:57:39Z-
dc.date.issued2019-
dc.identifier.issn2234-3806-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/173295-
dc.description.abstractBACKGROUND: The emergence of carbapenem resistance among gram-negative bacilli (GNB), mediated by carbapenemase production, has necessitated the development of a simple and accurate device for detecting minimum inhibitory concentrations (MICs) and resistance mechanisms, especially carbapenemase production. We evaluated the performance of the BD Phoenix NMIC-500 panel (BD Diagnostic Systems, Sparks, MD, USA) for antimicrobial susceptibility testing (AST) and carbapenemase-producing organism (CPO) detection. METHODS: We used 450 non-duplicate clinical GNB isolates from six general hospitals in Korea (409 Enterobacteriaceae and 41 glucose non-fermenting bacilli [GNFB] isolates). AST for meropenem, imipenem, ertapenem, ceftazidime, and ceftazidime/avibactam, and CPO detection were performed using the Phoenix NMIC-500 panel. Broth microdilution was used as the reference method for AST. The rates of categorical agreement (CA), essential agreement (EA), minor error (mE), major error (ME), and very major error (VME) were calculated in each antimicrobial. In addition, PCR and sequencing were performed to evaluate the accuracy of CPO detection by the BD Phoenix NMIC-500 panel, and the rate of correct identification was calculated. RESULTS: The CA rates were >90% for all antimicrobials tested with the Enterobacteriaceae isolates, except for imipenem (87.2%). The GNFB CA rates ranged from 92.7% to 100% for all antimicrobials. The ME rates were 1.7% for Enterobacteriaceae and 0% for GNFB. The panel identified 97.2% (243/250) of the carbapenemase-producing isolates. CONCLUSIONS: The BD Phoenix NMIC-500 panel shows promise for AST and CPO detection.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherKorean Society for Laboratory Medicine-
dc.relation.isPartOfANNALS OF LABORATORY MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnti-Bacterial Agents/pharmacology-
dc.subject.MESHBacillus/drug effects-
dc.subject.MESHBacillus/isolation & purification-
dc.subject.MESHBacterial Proteins/genetics-
dc.subject.MESHDrug Resistance, Bacterial/drug effects-
dc.subject.MESHDrug Resistance, Bacterial/genetics-
dc.subject.MESHEnterobacteriaceae/drug effects-
dc.subject.MESHEnterobacteriaceae/isolation & purification-
dc.subject.MESHGram-Negative Bacteria/isolation & purification*-
dc.subject.MESHMicrobial Sensitivity Tests/methods*-
dc.subject.MESHbeta-Lactamases/genetics-
dc.titlePerformance Evaluation of the Newly Developed BD Phoenix NMIC-500 Panel Using Clinical Isolates of Gram-Negative Bacilli-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.googleauthorByeol Yi Park-
dc.contributor.googleauthorDemiana Mourad-
dc.contributor.googleauthorJun Sung Hong-
dc.contributor.googleauthorEun-Jeong Yoon-
dc.contributor.googleauthorDokyun Kim-
dc.contributor.googleauthorHyukmin Lee-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.identifier.doi10.3343/alm.2019.39.5.470-
dc.contributor.localIdA04891-
dc.contributor.localIdA03286-
dc.contributor.localIdA03619-
dc.relation.journalcodeJ00164-
dc.identifier.eissn2234-3814-
dc.identifier.pmid31037866-
dc.subject.keywordAntimicrobial susceptibility testing-
dc.subject.keywordBD Phoenix NMIC-500 panel-
dc.subject.keywordCarbapenemase-producing organisms-
dc.subject.keywordPerformance-
dc.contributor.alternativeNameKim, Dokyun-
dc.contributor.affiliatedAuthor김도균-
dc.contributor.affiliatedAuthor이혁민-
dc.contributor.affiliatedAuthor정석훈-
dc.citation.volume39-
dc.citation.number5-
dc.citation.startPage470-
dc.citation.endPage477-
dc.identifier.bibliographicCitationANNALS OF LABORATORY MEDICINE, Vol.39(5) : 470-477, 2019-
dc.identifier.rimsid63866-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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