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부타놀 추출법을 이용한 2-thiothiazolidine-4-carboxilic acid와 thiocarbamide의 동시정량에 관한연구
DC Field | Value | Language |
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dc.contributor.author | 노재훈 | - |
dc.contributor.author | 원종욱 | - |
dc.date.accessioned | 2019-11-11T07:04:37Z | - |
dc.date.available | 2019-11-11T07:04:37Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 1226-4326 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/172522 | - |
dc.description.abstract | This study was conducted to supplement limit of previous study, The objectives of this study were to select optimal conditions of high performance liquid chromatography(HPLC) operation for detecting urinary 2-thiothiazolicline-4-carboxylic acid(TTCA) and thiocarbamide simultaneously, and to evaluate recovery rates for various liquid-liquid extration method of these metabolites, The results are as follows : 1. The urinary TTCA and thiocarbamide were separate sharply when flow rate is $0.7m{\ell}/min$, using a series $C_8$ and C18 column, 50 mM KH2PO4 : acetonitrile (93.5 : 6.5) and pH 3.5 as a mobile phase. The retention time was TTCA, $12.07{\pm}0.11$(mean±SD, n=06), thiocarbamide, $7.85{\pm}0.01$ (mean±SD, n=6), respectively. The calibration curve for TTCA and thiocarbamide was linear within the range 0.05 to $30{\mu}g/m{\ell}$. 2. By the liquid-liquid extration, butanol extration with $(NH_4)_2$ as a salting-out reagent was used as a simultaneous extration method for these metabolites in acid state, and recovery rates of this method are urinary TTCA, $49.6{\pm}17.7$ (mean±SD, n=16), thiocarbamide, $43,9{\pm}5.50$ (mean±SD, n=16), respectively 3. The precision(pooled coefficients of variation for 4 concentration) of the urinary thiocarbamide analysis was 0.03754 by butanol liquid-liquid extraction with $(NH_4)_2$ as a salting-out reagent, and TTCA was 0.04082 by ethyl acetate liquid-liquid extration with $(NH_4)_2$ as a salting out reagent The above results show that the butanol liquid-liquid extraction with $(NH_4)_2$ as a salting-out reagent in acid state, and using a series $C_8$ and C18 column, 50 mM KH2PO4 : acetonitrile (93.5 : 6.5) and pH 3.5 as a mobile phase are suitable for the analysis of urinary TTCA and thiocarbamide simultaneously. The detection limit of TTCA and thiocarbamide was about $0.17{\mu}g/m{\ell}$, $0.07{\mu}g/m{\ell}$. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 한국산업위생학회 | - |
dc.relation.isPartOf | Journal of Korean Society of Occupational and Environmental Hygiene (한국산업위생학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | 부타놀 추출법을 이용한 2-thiothiazolidine-4-carboxilic acid와 thiocarbamide의 동시정량에 관한연구 | - |
dc.title.alternative | Simultaneous analysis for 2-thiothiazolidine-4-carboxilic acid and thiocarbamide using butanol extraction method | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Preventive Medicine and Public Health (예방의학교실) | - |
dc.contributor.googleauthor | 이상회 | - |
dc.contributor.googleauthor | 송재석 | - |
dc.contributor.googleauthor | 윤영식 | - |
dc.contributor.googleauthor | 김치년 | - |
dc.contributor.googleauthor | 원종욱 | - |
dc.contributor.googleauthor | 노재훈 | - |
dc.contributor.localId | A01294 | - |
dc.contributor.localId | A02442 | - |
dc.relation.journalcode | J03170 | - |
dc.subject.keyword | 2-thiothiazolidine-4-carboxylic acid | - |
dc.subject.keyword | Thiocarbamide | - |
dc.subject.keyword | high performance liquid chromatography | - |
dc.subject.keyword | liquid-liquid extration | - |
dc.contributor.alternativeName | Roh, Jae Hoon | - |
dc.contributor.affiliatedAuthor | 노재훈 | - |
dc.contributor.affiliatedAuthor | 원종욱 | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 208 | - |
dc.citation.endPage | 222 | - |
dc.identifier.bibliographicCitation | Journal of Korean Society of Occupational and Environmental Hygiene (한국산업위생학회지), Vol.10(1) : 208-222, 2000 | - |
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