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배양된 쥐의 골아세포에서 전기장에 의한 Nitric Oxide의 생성

Other Titles
 Nitric Oxide Production of Rat Osteoblast Cells by Pulsed Electromagnetic Field Radiation 
 문성환  ;  이진우  ;  장준섭 
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.3(2) : 119-124, 2000 
Journal Title
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) 
Issue Date
Osteoblast ; Pulsed electromagenetic field ; Nitric oxide
Experimental study was conducted to prove the effect of pulsed electromagnetic field (PEMF) on the production of Nitric oxide (NO) from the cultured rat osteoblast-like cells. Calvarium of thirty Sprgue-Dawley rats was digested by sequential collagenase and cultured in-vitro. The osteoblast cell phenotype was confirmed by expression of osteoclacin by immunohistochemistry. PEMF was generated and applied to cultured osteoblast cells. Production of NO was measured by Greiss reaction. Ten minute exposure of PEMF to ostoeblast cell showed increased NO content at 24 and 48 hours(p<0.05). Cultures with different duration of PEMF exposure (10, 20, 30 60 minutes) demonstrated similar responses. In conclusion. this study proved that NO can be generated with PEMF which support the notion that NO can be a possible mediator of PEMF on bone metabolism.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Moon, Seong Hwan(문성환)
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
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