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HSP25-induced radioresistance is associated with reduction of apoptotic death: Involvement of Bcl-2 or cell cycle.

 Sang-Hee Park  ;  Hae-Nyun Cho  ;  Su-Jae Lee  ;  Tae-Hwan Kim  ;  Yeonhee Lee  ;  Young-Mee Park  ;  Yong J. Lee  ;  Chul-Koo Cho  ;  Seong-Yul Yoo  ;  Yun-Sil Lee 
 Radiation Research, Vol.154(4) : 421-428, 2000 
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Animals ; Apoptosis*/radiation effects ; Cell Cycle*/radiation effects ; Cell Cycle Proteins/metabolism ; Colony-Forming Units Assay ; Gamma Rays ; Gene Expression Regulation/radiation effects ; Genes, bcl-2 ; Heat-Shock Proteins* ; L Cells (Cell Line)/cytology ; L Cells (Cell Line)/metabolism ; L Cells (Cell Line)/radiation effects ; Mice ; Mimosine/pharmacology ; Neoplasm Proteins/biosynthesis ; Neoplasm Proteins/genetics ; Neoplasm Proteins/physiology* ; Oxidative Stress ; Proto-Oncogene Proteins c-bcl-2/biosynthesis ; Proto-Oncogene Proteins c-bcl-2/physiology* ; Radiation Tolerance/drug effects ; Radiation Tolerance/physiology* ; Recombinant Fusion Proteins/physiology ; Transfection
We previously demonstrated the protective effect of the small heat-shock protein against oxidative damage induced by tumor necrosis factor alpha. Here we have extended our studies of the possible role of Hsp25 in ionizing radiation-induced damage. For these studies, we transfected murine fibroblast L929 cells with the Hsp25 gene and selected three stably transfected clones. Hsp25 overexpression conferred radioresistance as detected by clonogenic survival and induction of apoptosis. Interestingly, the Hsp25-transfected cells showed an increase in the level of the anti-apoptosis molecule Bcl2. We also observed alterations of cell growth in the Hsp25-transfected cells. The cell cycle time of Hsp25-transfected cells was 3-4 h slower than that of vector-transfected control cells. Flow cytometry analysis of synchronized cells at late G(1) phase by mimosine treatment also showed the growth delay in Hsp25-overexpressing cells. In addition, reduced cyclin D1, cyclin A and Cdc2 levels and increased levels of Cdkn1a (also known as p21(Waf)) were observed in Hsp25-transfected cells, which probably caused the reduction in cell growth. In addition, synchronization by mimosine treatment only partially altered radioresistance in the Hsp25-transfected cells. Taken together, these data suggest that Hsp25-induced radioresistance is associated with growth delay as well as induction of Bcl2.
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1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yun Sil(이윤실)
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