318 668

Cited 828 times in

Long-term prevention of renal insufficiency, excess matrix gene expression, and glomerular mesangial matrix expansion by treatment with monoclonal antitransforming growth factor-beta antibody in db/db diabetic mice

DC Field Value Language
dc.contributor.author홍순원-
dc.date.accessioned2019-11-11T05:23:57Z-
dc.date.available2019-11-11T05:23:57Z-
dc.date.issued2000-
dc.identifier.issn0027-8424-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/171845-
dc.description.abstractEmerging evidence suggests that transforming growth factor-beta (TGF-beta) is an important mediator of diabetic nephropathy. We showed previously that short-term treatment with a neutralizing monoclonal anti-TGF-beta antibody (alphaT) in streptozotocin-diabetic mice prevents early changes of renal hypertrophy and increased matrix mRNA. To establish that overactivity of the renal TGF-beta system mediates the functional and structural changes of the more advanced stages of nephropathy, we tested whether chronic administration of alphaT prevents renal insufficiency and glomerulosclerosis in the db/db mouse, a model of type 2 diabetes that develops overt nephropathy. Diabetic db/db mice and nondiabetic db/m littermates were treated intraperitoneally with alphaT or control IgG, 300 microgram three times per week for 8 wk. Treatment with alphaT, but not with IgG, significantly decreased the plasma TGF-beta1 concentration without decreasing the plasma glucose concentration. The IgG-treated db/db mice developed albuminuria, renal insufficiency, and glomerular mesangial matrix expansion associated with increased renal mRNAs encoding alpha1(IV) collagen and fibronectin. On the other hand, treatment with alphaT completely prevented the increase in plasma creatinine concentration, the decrease in urinary creatinine clearance, and the expansion of mesangial matrix in db/db mice. The increase in renal matrix mRNAs was substantially attenuated, but the excretion of urinary albumin factored for creatinine clearance was not significantly affected by alphaT treatment. We conclude that chronic inhibition of the biologic actions of TGF-beta with a neutralizing monoclonal antibody in db/db mice prevents the glomerulosclerosis and renal insufficiency resulting from type 2 diabetes.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherNational Academy of Sciences-
dc.relation.isPartOfProceedings of the National Academy of Sciences of the United States of America-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Monoclonal/therapeutic use*-
dc.subject.MESHCarrier Proteins/genetics-
dc.subject.MESHDiabetes Mellitus, Type 2/complications*-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHExtracellular Matrix/drug effects*-
dc.subject.MESHExtracellular Matrix Proteins/biosynthesis-
dc.subject.MESHGlomerular Mesangium/drug effects*-
dc.subject.MESHGlomerular Mesangium/pathology-
dc.subject.MESHMice-
dc.subject.MESHMice, Mutant Strains-
dc.subject.MESHProtein-Serine-Threonine Kinases-
dc.subject.MESHReceptors, Cell Surface*-
dc.subject.MESHReceptors, Leptin-
dc.subject.MESHReceptors, Transforming Growth Factor beta/biosynthesis-
dc.subject.MESHRenal Insufficiency/etiology-
dc.subject.MESHRenal Insufficiency/prevention & control*-
dc.subject.MESHTransforming Growth Factor beta/immunology*-
dc.subject.MESHUp-Regulation-
dc.titleLong-term prevention of renal insufficiency, excess matrix gene expression, and glomerular mesangial matrix expansion by treatment with monoclonal antitransforming growth factor-beta antibody in db/db diabetic mice-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pathology (병리학교실)-
dc.contributor.googleauthorFuad N. Ziyadeh-
dc.contributor.googleauthorBrenda B. Hoffman-
dc.contributor.googleauthorDong Cheol Han-
dc.contributor.googleauthorM. Carmen Iglesias-de la Cruz-
dc.contributor.googleauthorSoon Won Hong-
dc.contributor.googleauthorMotohide Isono-
dc.contributor.googleauthorSheldon Chen-
dc.contributor.googleauthorTracy A. McGowan-
dc.contributor.googleauthorKumar Sharma-
dc.identifier.doi10.1073/pnas.120055097-
dc.contributor.localIdA04411-
dc.relation.journalcodeJ02550-
dc.identifier.eissn1091-6490-
dc.identifier.pmid10859350-
dc.contributor.alternativeNameHong, Soon Won-
dc.contributor.affiliatedAuthor홍순원-
dc.citation.volume97-
dc.citation.number14-
dc.citation.startPage8015-
dc.citation.endPage8020-
dc.identifier.bibliographicCitationProceedings of the National Academy of Sciences of the United States of America, Vol.97(14) : 8015-8020, 2000-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.