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Remission in models of type 1 diabetes by gene therapy using a single-chain insulin analogue

DC Field Value Language
dc.contributor.author김경섭-
dc.contributor.author이현철-
dc.date.accessioned2019-11-11T05:21:44Z-
dc.date.available2019-11-11T05:21:44Z-
dc.date.issued2000-
dc.identifier.issn0028-0836-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/171809-
dc.description.abstractA cure for diabetes has long been sought using several different approaches, including islet transplantation, regeneration of β cells and insulin gene therapy1. However, permanent remission of type 1 diabetes has not yet been satisfactorily achieved. The development of type 1 diabetes results from the almost total destruction of insulin-producing pancreatic β cells by autoimmune responses specific to β cells2,3,4,5,6. Standard insulin therapy may not maintain blood glucose concentrations within the relatively narrow range that occurs in the presence of normal pancreatic β cells7. We used a recombinant adeno-associated virus (rAAV) that expresses a single-chain insulin analogue (SIA), which possesses biologically active insulin activity without enzymatic conversion, under the control of hepatocyte-specific L-type pyruvate kinase (LPK) promoter, which regulates SIA expression in response to blood glucose levels. Here we show that SIA produced from the gene construct rAAV-LPK-SIA caused remission of diabetes in streptozotocin-induced diabetic rats and autoimmune diabetic mice for a prolonged time without any apparent side effects. This new SIA gene therapy may have potential therapeutic value for the cure of autoimmune diabetes in humans.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherNature Publishing Group-
dc.relation.isPartOfNature-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHBase Sequence-
dc.subject.MESHBlood Glucose/metabolism-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHDNA, Complementary-
dc.subject.MESHDNA, Recombinant/genetics-
dc.subject.MESHDNA, Recombinant/metabolism-
dc.subject.MESHDependovirus/genetics-
dc.subject.MESHDiabetes Mellitus, Experimental/therapy-
dc.subject.MESHDiabetes Mellitus, Type 1/therapy*-
dc.subject.MESHEscherichia coli-
dc.subject.MESHGene Expression-
dc.subject.MESHGenetic Therapy*-
dc.subject.MESHGenetic Vectors-
dc.subject.MESHGlucose Tolerance Test-
dc.subject.MESHHalf-Life-
dc.subject.MESHHepatocytes/metabolism-
dc.subject.MESHInsulin/analogs & derivatives-
dc.subject.MESHInsulin/genetics*-
dc.subject.MESHInsulin/secretion-
dc.subject.MESHLiver/metabolism-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred NOD-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPlasmids-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.titleRemission in models of type 1 diabetes by gene therapy using a single-chain insulin analogue-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)-
dc.contributor.googleauthorHyun Chul Lee-
dc.contributor.googleauthorSu-Jin Kim-
dc.contributor.googleauthorKyung-Sup Kim-
dc.contributor.googleauthorHang-Cheol Shin-
dc.contributor.googleauthorJi-Won Yoon-
dc.identifier.doi10.1038/35044106-
dc.contributor.localIdA00297-
dc.contributor.localIdA03301-
dc.relation.journalcodeJ02289-
dc.identifier.eissn1476-4687-
dc.identifier.pmid11100731-
dc.identifier.urlhttp://www.nature.com/articles/35044106-
dc.contributor.alternativeNameKim, Kyung Sup-
dc.contributor.affiliatedAuthor김경섭-
dc.contributor.affiliatedAuthor이현철-
dc.citation.volume23-
dc.citation.number408-
dc.citation.startPage483-
dc.citation.endPage488-
dc.identifier.bibliographicCitationNature, Vol.23(408) : 483-488, 2000-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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