Cited 219 times in
Remission in models of type 1 diabetes by gene therapy using a single-chain insulin analogue
DC Field | Value | Language |
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dc.contributor.author | 김경섭 | - |
dc.contributor.author | 이현철 | - |
dc.date.accessioned | 2019-11-11T05:21:44Z | - |
dc.date.available | 2019-11-11T05:21:44Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0028-0836 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171809 | - |
dc.description.abstract | A cure for diabetes has long been sought using several different approaches, including islet transplantation, regeneration of β cells and insulin gene therapy1. However, permanent remission of type 1 diabetes has not yet been satisfactorily achieved. The development of type 1 diabetes results from the almost total destruction of insulin-producing pancreatic β cells by autoimmune responses specific to β cells2,3,4,5,6. Standard insulin therapy may not maintain blood glucose concentrations within the relatively narrow range that occurs in the presence of normal pancreatic β cells7. We used a recombinant adeno-associated virus (rAAV) that expresses a single-chain insulin analogue (SIA), which possesses biologically active insulin activity without enzymatic conversion, under the control of hepatocyte-specific L-type pyruvate kinase (LPK) promoter, which regulates SIA expression in response to blood glucose levels. Here we show that SIA produced from the gene construct rAAV-LPK-SIA caused remission of diabetes in streptozotocin-induced diabetic rats and autoimmune diabetic mice for a prolonged time without any apparent side effects. This new SIA gene therapy may have potential therapeutic value for the cure of autoimmune diabetes in humans. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | Nature | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Blood Glucose/metabolism | - |
dc.subject.MESH | Cloning, Molecular | - |
dc.subject.MESH | DNA, Complementary | - |
dc.subject.MESH | DNA, Recombinant/genetics | - |
dc.subject.MESH | DNA, Recombinant/metabolism | - |
dc.subject.MESH | Dependovirus/genetics | - |
dc.subject.MESH | Diabetes Mellitus, Experimental/therapy | - |
dc.subject.MESH | Diabetes Mellitus, Type 1/therapy* | - |
dc.subject.MESH | Escherichia coli | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Genetic Therapy* | - |
dc.subject.MESH | Genetic Vectors | - |
dc.subject.MESH | Glucose Tolerance Test | - |
dc.subject.MESH | Half-Life | - |
dc.subject.MESH | Hepatocytes/metabolism | - |
dc.subject.MESH | Insulin/analogs & derivatives | - |
dc.subject.MESH | Insulin/genetics* | - |
dc.subject.MESH | Insulin/secretion | - |
dc.subject.MESH | Liver/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred NOD | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Plasmids | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.title | Remission in models of type 1 diabetes by gene therapy using a single-chain insulin analogue | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.googleauthor | Hyun Chul Lee | - |
dc.contributor.googleauthor | Su-Jin Kim | - |
dc.contributor.googleauthor | Kyung-Sup Kim | - |
dc.contributor.googleauthor | Hang-Cheol Shin | - |
dc.contributor.googleauthor | Ji-Won Yoon | - |
dc.identifier.doi | 10.1038/35044106 | - |
dc.contributor.localId | A00297 | - |
dc.contributor.localId | A03301 | - |
dc.relation.journalcode | J02289 | - |
dc.identifier.eissn | 1476-4687 | - |
dc.identifier.pmid | 11100731 | - |
dc.identifier.url | http://www.nature.com/articles/35044106 | - |
dc.contributor.alternativeName | Kim, Kyung Sup | - |
dc.contributor.affiliatedAuthor | 김경섭 | - |
dc.contributor.affiliatedAuthor | 이현철 | - |
dc.citation.volume | 23 | - |
dc.citation.number | 408 | - |
dc.citation.startPage | 483 | - |
dc.citation.endPage | 488 | - |
dc.identifier.bibliographicCitation | Nature, Vol.23(408) : 483-488, 2000 | - |
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